膀胱移行上皮Toll样受体分子诱导表达的实验研究

目的 了解膀胱移行上皮在大肠杆菌和卡介苗的不同刺激下Toll样受体分子(TLRs)的表达。方法 用不同剂量(菌数／细胞数比值)的大肠杆菌和卡介苗(BCG)分别刺激培养人膀胱移行上皮癌T24细胞，1h后以RT-PCR检测其TLR-2和TLR-4的mRNA水平。结果 膀胱癌T24细胞有TLR-2和TLR-4表达；大肠杆菌各剂量组T24细胞TLR-2和TLR-4的表达水平与未刺激组相比无显著差异；BCG刺激后T24细胞中TLR-2和TLR-4的表达水平随BCG剂量的增加而逐渐升高，在菌数／细胞数比值为10时达最高，随后有所降低。结论 膀胱移行上皮在大肠杆菌和BCG等不同微生物的刺激下，其Toll样受体分子的表达存在差异。

Study on the Inducible Expression of Toll-like Receptors in Human Bladder Cell Line

OBJECTIVE: To investigate and compare the expression of Toll-like receptors (TLRs) in human bladder cells under the stimulus of Escherichia coli (E. coli) versus that under the stimulus of Bacillus Calmette-Guérin (BCG). METHODS: Human bladder cancer cell line T24 was cultured for 1 hour under the stimuli of various doses (bacillus-cell ratio) of E. coli and BCG respectively. The levels of expression of TLR-2 and TLR-4 mRNA in T24 cells were assessed by RT-PCR method. RESULTS: By comparison with control, there was no difference observed on the expression of either TLR-2 or TLR-4 in T24 cells under the stimulus of E. coli. The expression level of either TLR-2 or TLR-4 was increased under the stimulus of BCG in a dose-dependent manner. The effects reached the highest level when the dose of BCG was 10 bacilli per cell. CONCLUSION: There exist different expression patterns of TLRs in bladder transitional cells under the different stimuli of E. coli and BCG respectively.