| Abstract: | The effect of prostaglandin E1 (PGE1) on fat mobilizing lipolysis in rat adipose tissue was studied in relation to the nutritional condition by following the release of glycerol during incubation in vitro. In fed tissues PGE1 at concentration of 0.01, 0.1, 1 and 10 μg/ml incubation medium significantly lowered the lipolysis to 64, 41, 48 and 68% of the basal rate (average of 6 expts.). The lipolytic rate was significantly higher with 10 than with 0.1 μg/ml of PGE1. The corresponding figures in adipose tissue from fasted rats were 90, 91, 86 and 95% respectively (average of 7 expts.). These average decreases were not statistically significant. Refeeding fasted rats with glucose one hour before the study restored the sensitivity of adipose tissue to the antilipolytic effect of PGE1. In rat fat the nutritional condition is thus of importance for the appearance of the antilipolytic effect of PGE1. The possible role of the tissue sensitivity to PGE1 in regulating fat mobilizing lipolysis during fasting was discussed. |
