| HPLC法测定盐酸异丙嗪注射液中维生素C的含量 |
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| 引用本文： | 刘春光,陈新善,武向锋.HPLC法测定盐酸异丙嗪注射液中维生素C的含量[J].解放军药学学报,2013(2):155-156,159. |
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| 作者姓名： | 刘春光 陈新善 武向锋 |
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| 作者单位： | [1]武警北京总队医院,北京100027 [2]总后勤部卫生部药品仪器检验所,北京100166 |
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| 摘 要： | 目的建立HPLC法测定盐酸异丙嗪注射液中维生素C的含量。方法采用Kromasil 100-5NH2分析色谱柱,甲醇-0.05 mol·L-1磷酸二氢钾溶液（磷酸调pH 3.0）（60∶40）为流动相,检测波长为243 nm;流速：1.0 ml·min-1;柱温：30℃。结果维生素C在0.010 01～0.1001 mg·ml-1的范围内线性关系良好（r=0.9997）,平均回收率为99.69%,RSD为0.52%（n=9）。结论本方法简便、测定结果准确,重复性好,可用于盐酸异丙嗪注射液中维生素C的含量测定。
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| 关 键 词： | 盐酸异丙嗪注射液 维生素C HPLC 含量测定 |
termination of Vitamin C in Promethazine Hydrochloride Injection by HPLC |
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| LIU Chun-guang,CHEN Xin-Shan,WU Xiang-feng.termination of Vitamin C in Promethazine Hydrochloride Injection by HPLC[J].Pharmaceutical Journal of Chinese People's Liberation Army,2013(2):155-156,159. |
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| Authors: | LIU Chun-guang CHEN Xin-Shan WU Xiang-feng |
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| Institution: | Beijing Corps Hospital, Chinese Peopleg Armed Police Forces,Beijing 100027, China; Institute for Drug and Instrument Control, Health Dept, GLD of PLA, Beijing 100166, China |
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| Abstract: | Objective To establish an HPLC method for determination of vitamin C in promethazine hydro- chloride injection. Methods Kromasil 100-5NH2 column was used as the chromatographic column, and the mobile phase was composed of methanol-0.05 mol .L-1 potassium dihydrogen phosphate solution（ the pH value of solution adjusted to 3.0 by phosphoric acid） （60： 40）. The flow rate was 1.0 ml -min-1 and detection wavelength was 243 nm. The column temperature was 30 C. Results The calibration curves of vitamin C showed a good linear relation- ship over the range of 0. 010 01-0. 1001 mg ml -1 （ r = 0.9997 ）. The average recovery of vitamin C was 99.69% and RSD was 0.52% （ n = 9）. Conclusion This method is simple, accurate andwith good reproducibility. It can be used for determination of vitamin C in promethazine hydrochloride injection. |
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| Keywords: | promethazine hydrochloride injection vitamin C HPLC determination |
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