| HPLC法测定肾石消胶囊中绿原酸的含量 |
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| 引用本文： | 吴海霞,郭迎霞,曹宇峰. HPLC法测定肾石消胶囊中绿原酸的含量[J]. 解放军药学学报, 2013, 0(3): 242-243 |
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| 作者姓名： | 吴海霞 郭迎霞 曹宇峰 |
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| 作者单位： | [1]邯郸市食品药品检验中心,河北邯郸056001 [2]邯郸市中医院,河北邯郸056001 |
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| 摘 要： | 目的建立用RP-HPLC法测定肾石消胶囊中绿原酸含量的方法。方法以乙腈-0.4%冰醋酸（11∶89）为流动相,SHIMADZU VP-ODS（5μm,250 mm×4.6 mm）为固定相,检测波长为326 nm,流速1.0 ml.min-1。结果绿原酸进样量在0.0828～0.828μg范围内线性关系良好,r=0.9994（n=5）,平均回收率为99.6%,RSD为0.4%。结论该法结果准确、方法简便、分离度高可用于该制剂的质量控制。
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| 关 键 词： | HPLC 肾石消胶囊 绿原酸 |
Determination of Chlorogenic Acid in Shenshixiao Capsules by HPLC |
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| WU Hai-xia,GUO Ying-xia,CAO Yu-feng. Determination of Chlorogenic Acid in Shenshixiao Capsules by HPLC[J]. Pharmaceutical Journal of Chinese People's Liberation Army, 2013, 0(3): 242-243 |
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| Authors: | WU Hai-xia GUO Ying-xia CAO Yu-feng |
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| Affiliation: | Handan Institute of Food and Drug Control,Handan 056001 ,China; Traditional Chinese Medicine Hospital of Handan, Handan 056001, China |
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| Abstract: | Objective To establish an HPLC method for determination of Chlorogenic acid in Shenshixiao capsules. Methods SHIMADZU VP-ODS column （4.6 mm× 250 mm,5 μm） was used with acetonitrile-0.04% phosphoric acid（11：89） as the mobile phase at a flow rate of 1.0 ml .min-1. The detection wavelength was 326 nm. Results The linear relationship was good when paracetamol was 0. 0828 -0.828 μg（ r = 0. 9994 ）. The average recovery was 99.6%, RSD = 0.4%. Conclusion This method is simple, accurate for quantity control. |
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| Keywords: | HPLC Shenshixiao capsules chlorogenic acid |
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