动员血管内皮祖细胞促进真皮支架移植区的血管新生

背景：近年研究发现促红细胞生成素可以动员自体骨髓中的血管内皮祖细胞至外周血,趋化其至创面,促进创面血管新生。目的：观察促红细胞生成素对血管内皮祖细胞动员效果的时效关系。方法：不同剂量促红细胞生成素通过腹腔注射7d动员小鼠血管内皮祖细胞,以注射生理盐水作为对照,采用流式细胞仪定点检测外周血中内皮祖细胞数量以比较动员效果,优选安全有效的促红细胞生成素动员剂量,并观察促红细胞生成素动员内皮祖细胞对脱细胞猪皮移植区血管新生的影响。结果与结论：在开始注射后1,3,5,7,10,14d等6个时间点内,生理盐水对照组外周血内皮祖细胞无明显变化,促红细胞生成素动员组外周血内皮祖细胞逐渐增加,于7d达到高峰。高剂量促红细胞生成素动员组内皮祖细胞比例较低剂量组增加明显,促红细胞生成素动员组促进脱细胞猪皮移植区血管新生的效果明显强于对照组。结果可见,促红细胞生成素连续7d腹腔注射能增加外周血内皮祖细胞的数量,在动员后第7天时达到高峰,效果成剂量依赖性,并能促进脱细胞猪皮移植区血管新生。

Angiogenesis of dermal scaffold transplantation zone promoted by moboilizing endothelial progenitor cells

BACKGROUND： In recent years, erythropoietin is found to mobilize autologous vascular endothelial progenitor cells from the bone marrow to peripheral blood, which are moved to the wound and promote wound angiogenesis and wound healing ultimately. OBJECTIVE： To observe the effect of erythropoietin on the mobilization of vascular endothelial progenitor cellsMETHODS： The vascular endothelial progenitor cells were mobilized by intraperitoneal injection of erythropoietin with different concentrations for 7 days, and the cells injected with normal saline were as control. The number of endothelial progenitor cells in the peripheral blood was detected with flow cytometry to compare the mobilization effect. The safe and effective erythropoietin mobilization dose was selected to observe the effect of erythropoietin- mobilized endothelial progenitor cells on the angiogenesis of the dermal scaffold transplantation zone. RESULTS AND CONCLUSION： In 1, 3, 5, 7, 10 and 14 days after injection, there was no significant change in the number of endothelial progenitor cells in the peripheral blood of the normal saline group, while the number of endothelial progenitor cells in the peripheral blood of the erythropoietin mobilization group was increased gradually and reached peak at 7 days after injection. The proportion of endothelial progenitor cells was significantly increased in the high-dose erythropoietin mobilization group when compared with the low-dose erythropoietin mobilization group. The erythropoietin mobilization group had better effect on the angiogenesis in the dermal scaffold transplantation zone than the control group. Consecutive intraperitoneal injection of erythropoietin for 7 days can increase the number of endothelial progenitor cells in the peripheral blood that reaches peak at 7 days after mobilization. The mobilization effect is in a dose-dependent manner, and the injection can promote the angiogenesis in the dermal scaffold transplantation area.