| HPLC法同时测定川参通注射液中丹参素和原儿茶醛的含量 |
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| 引用本文： | 赵晶,曹红.HPLC法同时测定川参通注射液中丹参素和原儿茶醛的含量[J].解放军药学学报,2013(2):141-143. |
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| 作者姓名： | 赵晶 曹红 |
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| 作者单位： | [1]第二军医大学药学院,上海200433 [2]总后勤部卫生部药品仪器检验所,北京100166 |
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| 摘 要： | 目的建立HPLC法同时测定川参通注射液中丹参素和原儿茶醛含量的方法。方法色谱柱：Discovery C18色谱柱（250 mm×4.6 mm,5μm）,柱温为35℃;流动相为乙腈和0.4%冰醋酸水溶液,梯度洗脱;流速：1.0 ml·min-1;检测波长：281 nm,进样量为5μl。结果丹参素在69.1～1382.0μg·ml-1（r=0.9999）范围内线性关系良好,平均回收率为99.36%（RSD=0.56%）;原儿茶醛在5.3～105.6μg·ml-1（r=0.9998）范围内线性关系良好,平均回收率为98.13%（RSD=1.48%）。结论本方法结果准确可靠,重复性好,操作简便。
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| 关 键 词： | HPLC 川参通注射液 丹参素 原儿茶醛 |
Simultaneous Determination of Danshensu and Protocatechuic Aldehyde in Chuanshentong Injection by HPLC |
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| ZHAO Jing,CAO Hong.Simultaneous Determination of Danshensu and Protocatechuic Aldehyde in Chuanshentong Injection by HPLC[J].Pharmaceutical Journal of Chinese People's Liberation Army,2013(2):141-143. |
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| Authors: | ZHAO Jing CAO Hong |
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| Institution: | Department of Pharmacy,Second Military Medical University,Shanghai 200433,China; Institute for Drug and Instrument Control, Health Dept. ,GLD of PLA,Beijing 100166,China |
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| Abstract: | Objective To establish a method for determining danshensu and protocatechuic aldehyde in Chuanshentong injection by HPLC. Methods The chromatographic column was discovery Cls （ 250 mm ×4.6 mm, 5 μm）. The mobile phase consisted of acetonitrile and 0.4% acetic acid in gradient elution at a flow rate of 1.0 ml .min-1. The detection wavelength was at 281 nm, the column temperature was 35% and the injection volume was 5 1. Results Danshensu showed a good linear relationship within the range of 69.1-1382.0 μg .ml-1 （ r = 0. 9999） and the average recovery was 99.36% （RSD = 0.56% ）. Protocatechuic aldehyde showed a good linear relationship within the range of 5.3-105.6 μg ml-1（ r = 0. 9998 ） and the average recovery was 98.13% （RSD = 1.48% ）. Conclusion This method is reliable, simple and user-friendly. |
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| Keywords: | HPLC Chuanshentong injection danshensu protocatechuic aldehyde |
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