壳聚糖／鞣花酸／红细胞膜脂止血复合海绵的制备与评价

背景：部分文献报道壳聚糖对严重创伤的止血效果有限，因此以壳聚糖为基础止血剂的促凝血活性还有待进一步增强。目的：制备一种新型的壳聚糖，鞣花酸／红细胞膜脂复合海绵，评价其促凝血活性和细胞毒性。方法：通过冻干法制备壳聚糖海绵和壳聚糖乙酸盐海绵，然后再通过静电吸附法制备壳聚糖，鞣花酸，红细胞膜脂复合海绵。血浆复钙时间法观察3种海绵的促凝血活性，并检测3种海绵对SD大鼠肝脏的止血效果及对L929细胞的毒性。结果与结论：壳聚糖，鞣花酸，红细胞膜脂复合海绵组的血浆复钙时间、出血时间、失血量均显著少于壳聚糖海绵组和壳聚糖乙酸盐海绵组（P〈0．01）。细胞实验显示壳聚糖，鞣花酸，红细胞膜脂复合海绵无细胞毒性。说明壳聚糖，鞣花酸，红细胞膜脂复合海绵具有良好的促凝血活性且无细胞毒性。

Preparation and evaluation of chitosan/ellagic acid/erythrocyte membrane lipid hemostaUc composite sponge

BACKGROUND： Some previous studies have indicated that the hemostatic effect of chitosan is limited when dealing with severe injuries. Therefore, the procoagulant activity of chitosan-based hemostatic agents needs to be enhanced. OBJECTIVE： To prepare a novel chitosan/ellagic acid/erythrocyte membrane lipid composite sponge and to evaluate its procoagulant activity and cytotoxicity.METHODS： Chitosan sponge and chitosan acetate sponge were prepared by freeze-drying method. Then chitosan/ellagic acid/erythrocyte membrane lipid composite sponge was prepared by electrostatic adsorption method. Procoagulant activity of the chitosan, chitosan acetate, and chitosan/eilagic acid/erythrocyte membrane lipid sponges was evaluated by the plasma recalcification time method. Hemostatic effect of these sponges was evaluated in the Sprague Dawiey rat liver bleeding model, and the cytotoxicity to L929 cell line was evaluated. RESULTS AND CONCLUSION： The plasma recalcification time, bleeding time and blood loss of the chitosan/ellagic acid/erythrocyte membrane lipid composite sponge group were significantly lower than those of the chitosan sponge and chitosan acetate sponge groups （P 〈 0.01）. Cell culture experiment showed that the chitosan/ellagic acid/erythrocyte membrane lipid composite sponge did not have cytotoxicity. The results indicate that the chitosan/ellagic acid/erythrocyte membrane lipid composite sponge has the enhanced procoagulant activity and non-cytotoxicity.