The attenuating effect of aqueous extract of licorice on bleomycin-induced pulmonary fibrosis in mice |
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| Authors: | Mehri Ghorashi Mohammad Ali Rezaee Mohammad Jafar Rezaie Mehdi Mohammadi Ali Jalili |
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| Affiliation: | 1. Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran;2. Department of Immunology and Hematology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran;3. Department of Medical Laboratory Sciences, Faculty of Paramedicine, Kurdistan University of Medical Sciences, Sanandaj, Iran;4. Department of Anatomy, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran;5. Department of Immunology and Hematology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran |
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| Abstract: | Licorice has immunomodulatory and anti-asthmatic properties. This study aims to test the effect of licorice on pulmonary fibrosis (PF). PF was induced in mice through bleomycin instillation. One group of the mice received bleomycin (positive control), another one received phosphate-buffered saline and the other two received 300?mg/kg of the extracts for 20 days after the bleomycin instillation. Hematoxylin-eosin and Masson’s staining were used in the lung tissues. The expression of α-SMA, TGF-β1, IL-1β, and TNF-α were evaluated using reverse-transcriptase-polymerase-chain-reaction. Bleomycin led to tissue-degenerative changes, collagen deposition, and overexpression of the genes in the lung tissues. In the mice receiving the aqueous extract, the weight gain was higher (p?p?
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| Keywords: | Pulmonary fibrosis bleomycin licorice cytokine mice |
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