Grape seed extract (GSE) modulates campylobacter pro-inflammatory response in human intestinal epithelial cell lines

The main aim of this study was to investigate the effect of Grape seed extract (GSE) on Campylobacter induced-cytokine production by human intestinal cell lines. With this purpose, Caco-2 and HT-29 cells were challenged with culture supernatants from several strains of C. jejuni and levels of secreted IL-6, IL-8, and MCP-1 were measured. Cytokine production was higher in HT-29 than in Caco-2 cells, showing different levels of secretion depending of the epithelial cells origin. Also, infective isolates coming from campylobacteriosis patients rendered higher levels of pro-inflammatory cytokines. Co-treatment and pre-treatment of HT-29 cells with GSE and C. jejuni significantly reduced cytokines production in a dose-dependent manner. These results make this natural product a putative nutritional tool for use in the treatment of campylobacteriosis, which could contribute to improve disease prevention or reduce disease severity.