邻甲氧基肉桂酸抗衰老活性及机制研究

目的 基于秀丽隐杆线虫（以下简称线虫）模型研究邻甲氧基肉桂酸（OMA）体内抗衰老活性及机制。方法 研究OMA对野生型线虫体长、头部摆动频率、脂褐素含量、抗氧化应激能力的影响，评价其体内抗衰老活性；利用线虫转基因株系TJ356和CF1553模型，通过衰变加速因子-16（DAF-16）核转位实验、超氧化物歧化酶-3（SOD-3）融合绿色荧光蛋白（GFP）荧光检测实验探究OMA抗衰老作用的分子信号通路。结果 与对照组比较，OMA可以抑制线虫的体长（P<0.001）、降低其头部摆动频率、减缓线虫体内脂褐素沉积（P<0.001）、提高线虫在氧化应激条件下的寿命（P<0.05）；同时能激活DAF-16转录因子，促进线虫DAF-16核转位，上调线虫体内SOD-3蛋白表达水平（P<0.001）。结论 OMA具有良好的抗衰老活性，其作用机制可能与胰岛素/胰岛素样生长因子-1（IIS）信号通路有关，通过增强DAF-16活性并上调其下游SOD-3蛋白的表达发挥抗衰老作用。

Anti-aging Activity and Mechanism of o-Methoxycinnamic Acid

Objective To study the anti-aging activity and mechanism of o-methoxycinnamic acid in vivo based on the model of Caenorhabditis elegans.Methods The effects of o-methoxycinnamic acid on body length, head swinging frequency, lipofuscin content, and antioxidant ability of C. elegans were studied to evaluate its anti-aging activity in vivo. With the transgenic strains TJ356 and CF1553 of C. elegans as the animal models, the signaling pathway of the anti-aging effect of o-methoxycinnamic acid was predicted via decay-accelerating factor 16 (DAF-16) nuclear translocation assay and fluorescent aging marker superoxide dismutase 3 (SOD-3) ::green fluorescent protein (GFP).Results o-Methoxycinnamic acid shortened the body, reduced the head swing frequency, slowed down the lipofuscin deposition, and improved the life span of nematodes under oxidative stress. At the same time, it activated DAF-16, promoted the nuclear translocation of DAF-16, and up-regulated the expression of SOD-3 in nematodes.Conclusion o-Methoxycinnamic acid has good anti-aging activity, which may be related to insulin/insulin-like growth factor-1 (IIS) signaling pathway. It can achieve anti-aging effect by enhancing the activity of DAF-16 and up-regulating the expression of the downstream gene SOD-3.