胰腺癌中CIP2A与p-Akt、N-cadherin、MMP-9的表达及其临床意义

目的:探讨胰腺癌组织中蛋白磷酸酶2A癌性抑制因子(CIP2A)、p-Akt、N-cadherin及MMP-9蛋白的表达及其临床意义。方法:采用免疫组织化学法检测42例胰腺癌及癌旁组织中CIP2A、p-Akt、N-cadherin和MMP-9的表达情况,分析其与临床病理因素的关系。结果:CIP2A在胰腺癌及癌旁组织中的表达率分别为71.4%、7.7%,差异有统计学意义(P0.05);p-Akt在胰腺癌及癌旁组织中的表达率分别为66.7%、11.5%,差异有统计学意义(P0.05)。N-cadherin在胰腺癌及癌旁组织中的表达率分别为59.5%、7.7%,差异有统计学意义(P0.05)。MMP-9在胰腺癌组织及癌旁组织中的表达率分别为61.9%、15.4%,差异有统计学意义(P0.05)。CIP2A、p-Akt在胰腺癌组织中的表达与分化程度、TNM分期、淋巴结转移明显相关(均P0.05),N-cadherin、MMP-9在胰腺癌组织中的表达与分化程度、TNM分期、神经浸润、淋巴结转移明显相关(P0.05)。胰腺癌组织中,CIP2A和p-Akt、N-cadherin和MMP-9的表达均呈正相关(均P0.05);p-Akt与N-cadherin及MMP-9的表达均呈正相关(P0.05);N-cadherin与MMP-9的表达呈正相关(P0.05)。结论:CIP2A参与了胰腺癌恶性进展,可能通过PI3K/Akt信号通路诱导上皮间质转化,促进胰腺癌增殖、侵袭和转移。CIP2A有望成为治疗胰腺癌的一个靶向治疗基因。

Expression and signifance of CIP2A,p-Akt,N-cadherin and MMP-9 in pancreatic carcinoma

Objective：To study the expressions of ClP2A,p-Akt,N-cadherin and MMP-9 in pancreatic carcinoma and their clinical significance.Methods：The expressions of CIP2A,p-Akt,N-cadherin and MMP-9 proteins were tested by immunohistochemistry in 42 cases of pancreatic carcinomas and adjacent paracancerous tissues.Results：The positive rate of CIP2A in pancreatic carcinomas was significantly higher than adjacent paracancerous tissues （71.4％ vs 7.7％,P＜0.05）.Significant differences were also observed in the expression rate of p-Akt between the patients with pancreatic carcinomas and paracancerous tissues （66.7％ vs 11.5％,P＜0.05）.The positive rate of N-cadherin in pancreatic carcinomas and paracancerous tissues （59.5％ vs 7.7％,P＜0.05）.Significant differences were also observed in the expression rate of MMP-9 between pancreatic carcinomas and paracancerous tissues （61.9％ vs 15.4％,P＜0.05）.The differences of the expression of CIP2A and p-Akt in pancreatic carcinoma of tumor differentiation,TNM stage and lymph node metastasis were significant （all P＜0.05）.The differences of the expression of N-cadherin and MMP-9 in pancreatic carcinoma of tumor differentiation,TNM stage,neural invasion and lymph node metastasis were also significant （all P＜0.05）.Significantly positive correlation was found between the expression of ClP2A and the others （p-Akt,N-cadherin and MMP-9） by using spearman correlation analysis （all P＜0.05）.Significantly positive correlation was found between the expression of p-Akt and the others （N-cadherin and MMP-9） by using spearman correlation analysis（all P＜0.05）.Significantly positive correlation was found between the expression of N-cadherin and MMP-9 by using spearman correlation analysis （P＜0.05）.Conclusion：CIP2A was involved in the development of pancreatic carcinoma and may promote EMT through the PI3K/Akt pathway.The data identified CIP2A as a critical oncoprotein involved in cell proliferation,invasion and metastasis,which coul