免疫吸附法纯化人胚嗅鞘细胞的实验研究

目的:探索分离培养人胚嗅鞘细胞以及纯化的方法。方法:分离人胚嗅球,采用胰酶消化获取单细胞悬液,应用含Forskolin、BPE的DMEM/F12培养基,接种于p75包被的培养皿,培养2周后细胞行免疫组化染色鉴定。结果:培养细胞形态多为2￣4极细胞,成纤维细胞少,嗅鞘细胞纯度非常高,嗅鞘细胞标志物p75阳性。结论:嗅鞘细胞体外分离培养条件要求极高,应用p75免疫吸附纯化培养的嗅鞘细胞效果好。

The experimental study of purification of olfactory ensheathing cells from human embryo with immune adsorption in vitro

Objective:To explore the methods of fractional cultivation and purification of olfactory ensheathing cells (OECs) from human embryo with immune adsorption in vitro. Method:The olfactory bulb was separated from human embryo and monoplast suspension was harvested after trypsinization.Then the cells were seed in the culture dish custodited by p75 with DMEM/F12 medium containing Forskolin and BPE. Two weeks later, the cells were characterized by immunohistochemical staining. Result:The shape of the cultured cells was mostly for 2~4 poles and there were few fibroblasts in them.The purity coefficient of OECs in these cultured cells was very high and the marker of OECs, p75 was positive in immunohistochemical staining. Conclusion:The condition of fractional cultivation of OECs was relatively harsh.The effect of purification the of cultured OECs with the method of immune adsorption by p75 was fine.