淋巴细胞亚群及血细胞抗体检测在再生障碍性贫血中的临床意义

目的：探讨再生障碍性贫血患者外周血淋巴细胞亚群及血细胞抗体水平的变化及临床意义。方法采用流式细胞仪检测25例再生障碍性贫血患者（重型再生障碍性贫血7例，非重型再生障碍性贫血18例）及20例健康志愿者（对照）外周血淋巴细胞亚群（CD3＋、CD3＋ CD4＋、CD3＋ CD8＋、CD4＋／CD8＋、CD3－CD19＋、CD3－CD16＋ CD56＋）及血细胞抗体（抗血小板抗体、抗红细胞抗体及抗粒细胞抗体）。结果再生障碍性贫血组患者CD4＋ T 淋巴細胞百分比、CD4／CD8比值，CD56＋百分比明显降低（P＜0．05）；血细胞抗体（血小板抗体、红细胞抗体、粒细胞抗体）阳性率明显高于对照组（P＜0．05）。结论再生障碍性贫血患者细胞免疫功能异常，同时血细胞膜上抗体阳性率高，提示体液免疫也存在异常。

Clinical significance of detecting lymphocyte subsets and hematocyte antibody in aplastic anemia

Objective To investigate of the change of peripheral blood lymphocyte subsets and blood cell antibodies in aplas‐tic anemia and its clinical significance .Methods The lymphocyte subsets CD3+ ,CD3+ CD4+ ,CD3+ CD8+ ,CD4+ /CD8+ ,CD3-CD19+ ,CD3-CD16+CD56+ from 25 aplastic anemia(including SAA 7 cases ,NSAA18 cases) ,and hematocyte antibody detection (granulocyte ,erythrocyte ,platelet cytomembrane) and 20 controls were examined by flow cytometry .Results CD4+ T lymphocyte percentage ,CD4/CD8 ratio ,the percentage of CD56+ in aplastic anemia patients significantly decreased(P<0 .05) .Blood cell anti‐bodies (platelet antibody ,erythrocyte antibody ,granulocyte antibody) were significantly higher than the control group (P<0 .05) . Conclusion Aplastic anemia cellular immune function in patients is abnormal ,and antibody positive rate is high ,and it prompt that humoral immunity is also abnormal .