紧密连接蛋白1和血管内皮生长因子C蛋白与大肠癌的关系研究

目的：探讨紧密连接蛋白1（Claudin‐1）与血管内皮生长因子C（VEGF‐C）在大肠癌组织中的表达情况及二者间的相互关系。方法对该院病理科收集的50例大肠癌组织、癌旁组织及50例正常大肠组织采用免疫组织化学S P法检测，根据患者的年龄、性别、是否发生淋巴结转移、浸润深度、临床分期进行综合比较，分析Claudin‐1和VEGF‐C蛋白在大肠癌组织中表达的相关性。结果大肠癌组织的Claudin‐1、VEGF‐C阳性率显著高于癌旁组织和正常大肠组织（χ2＝32．270，P＝0．000；χ2＝41．209，P＝0．000）；癌旁组织的 Claudin‐1、VEGF‐C阳性率显著高于正常大肠组织（χ2＝7．294，P＝0．007；χ2＝5．741，P＝0．017）。大肠癌组织Claudin‐1的表达与分化程度、浸润程度、TNM 分期、淋巴结转移具有密切关系（P＜0．05），大肠癌组织VEGF‐C的表达与浸润程度、TNM分期、淋巴结转移、肿瘤大小具有密切关系（P＜0．05）。大肠癌Claudin‐1、VEGF‐C表达具有显著的正相关关系（χ2＝10．953，P＝0．001，r＝0．468）。结论大肠癌组织的Claudin‐1、VEGF‐C表达显著高于癌旁组织和正常大肠组织，大肠癌组织的Claudin‐1、VEGF‐C表达与临床分期等因素具有一定的关系。

Relationship of Claudin-1 and VEGF-C protein with colorectal cancer

Objective To investigate the expression of tight junction protein 1(Claudin‐1) and vascular endothelial growth factor C(VEGF‐C) in colorectal carcinoma tissues and the relationship between them .Methods Totally 50 cases of colorectal carci‐noma ,paracancerous tissues and 50 cases of normal colorectal tissues from Department of Pathology were detected by immunohisto‐chemistry SP method ,according to the patient′s age ,gender ,lymph node metastasis ,depth of invasion ,clinical stage for comprehen‐sive comparison ,and analyze the Claudin 1 and VEGF‐C protein expression in colorectal cancer tissues .Results The positive rates of Claudin‐1 ,VEGF‐C in colorectal cancer tissues was significantly higher than that in paracancerous tissue and normal colorectal tissues (χ2 =32 .270 ,P=0 .000;χ2 =41 .209 ,P=0 .000) ,the positive rate of Claudin‐1 ,VEGF‐C in paracancerous cancer tissues was significantly higher than that in normal colorectal tissues (χ2 =7 .294 ,P=0 .007 ;χ2 = 5 .741 ,P=0 .017) .The expression of colorectal carcinoma Claudin‐1 had close relationship with differentiation ,invasion degree ,TNM stage ,lymph node metastasis(P<0 .05) ,the expression of VEGF‐C had colse relatons with infiltration degress ,TNM stage ,lymph node metastasis ,tumor size(P<0 .05) .The expression of colorectal cancer Claudin‐1 ,VEGF‐C had significantly positive correlation (χ2 =10 .953 ,P= 0 .001 ,r=0 .468) .Conclusion The expression of colorectal cancer Claudin‐1 ,VEGF‐C was significantly higher than that in paracancerous tis‐sue and normal colorectal tissue ,the expression of Claudin‐1 ,VEGF‐C had a certain relationship with clinical staging .