电针预处理抑制癫痫持续状态诱发的海马区神经元凋亡研究

目的：观察电针预处理对癫痫持续状态（status epilepticus,SE）诱发的海马区神经元凋亡的影响。方法：20只SD大鼠被随意分为4组：对照组、癫痫组、电针组、电针对照组,电针组和电针对照组给予电针预处理3周后,按经典方法建立锂-匹罗卡品大鼠癫痫模型,用TUNEL法及免疫荧光法观察海马区神经元的凋亡。结果：（1）与对照组比较,癫痫组和电针对照组海马CA1/CA3区TUNEL及Caspase-3阳性的神经元明显增多（P〈0.01,n=5）;（2）与癫痫组和电针对照组相比,电针预处理减少了海马CA1/CA3区TUNEL及Caspase-3阳性神经元的数量（P〈0.05,n=5）。结论：电针百会及大椎穴预处理,可以减低SE诱发的海马区神经元的凋亡。

Electroacupuncture Pretreatment Suppressed Hippocampal Neuron Apoptosis Induced by Experimental Epileptic Model in Rats

Objective：To observe the suppressive effect of electroacupuncture（EA）pretreatment on hippocampal neuron apoptosis induced by Li-pilocarpine status epilepticus.Methods：20 SD rats were randomly divided into four groups：Control group,EA group,Pilo group and Placebo group.Pilo group and Placebo group rats were pretreated with electro-acupunture for 3 weeks.Li-pilocarpine status epileptic model was established according to the previous schedule.TUNEL and immunofluoresence were employed to observe the damaged and surviving neurons in hippocampus.Results：（1）Compared with control group,the Pilo and Placebo groups’ rats exhibited a large number of TUNEL-positive and caspase-3 positive cells in the CA1/CA3 region（P〈0.01,n=5）.（2）EA pretreatment at Baihui and Dazhui acupoints decreased the total number of TUNEL-positive and caspase-3 positive cells in CA1/CA3 region of hippocampus in comparison with Pilo and Placebo groups（P〈0.05,n=5）.Conclusion：EA pretreatment at Dazhui and Baihui acupoints suppressed the SE induced hippocampal neuron apoptosis in rats.