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电针预处理抑制癫痫持续状态诱发的海马区神经元凋亡研究
引用本文:杨俊,黄远桂,徐江涛. 电针预处理抑制癫痫持续状态诱发的海马区神经元凋亡研究[J]. 辽宁中医学院学报, 2011, 0(3): 104-106
作者姓名:杨俊  黄远桂  徐江涛
作者单位:[1]兰州军区乌鲁木齐总医院神经内科,新疆乌鲁木齐830000 [2]第四军医大学西京医院神经内科癫痫中心,陕西西安710020
摘    要:目的:观察电针预处理对癫痫持续状态(status epilepticus,SE)诱发的海马区神经元凋亡的影响。方法:20只SD大鼠被随意分为4组:对照组、癫痫组、电针组、电针对照组,电针组和电针对照组给予电针预处理3周后,按经典方法建立锂-匹罗卡品大鼠癫痫模型,用TUNEL法及免疫荧光法观察海马区神经元的凋亡。结果:(1)与对照组比较,癫痫组和电针对照组海马CA1/CA3区TUNEL及Caspase-3阳性的神经元明显增多(P〈0.01,n=5);(2)与癫痫组和电针对照组相比,电针预处理减少了海马CA1/CA3区TUNEL及Caspase-3阳性神经元的数量(P〈0.05,n=5)。结论:电针百会及大椎穴预处理,可以减低SE诱发的海马区神经元的凋亡。

关 键 词:癫痫  凋亡  电针  癫痫持续状态  认知损害

Electroacupuncture Pretreatment Suppressed Hippocampal Neuron Apoptosis Induced by Experimental Epileptic Model in Rats
YANG Jun,HUANG Yuan-gui,XU Jiang-tao. Electroacupuncture Pretreatment Suppressed Hippocampal Neuron Apoptosis Induced by Experimental Epileptic Model in Rats[J]. Journal of Liaoning College of Traditional Chinese Medicine, 2011, 0(3): 104-106
Authors:YANG Jun  HUANG Yuan-gui  XU Jiang-tao
Affiliation:1(1.Department of Neurology,Xinjiang Urumqi Military General Hospital,Urumqi 830000,Xinjiang,China;2.Department of Neurology,Xijing Hospital,The Fourth Military Medical University,Xi’an 710020,Shaanxi,China)
Abstract:Objective:To observe the suppressive effect of electroacupuncture(EA)pretreatment on hippocampal neuron apoptosis induced by Li-pilocarpine status epilepticus.Methods:20 SD rats were randomly divided into four groups:Control group,EA group,Pilo group and Placebo group.Pilo group and Placebo group rats were pretreated with electro-acupunture for 3 weeks.Li-pilocarpine status epileptic model was established according to the previous schedule.TUNEL and immunofluoresence were employed to observe the damaged and surviving neurons in hippocampus.Results:(1)Compared with control group,the Pilo and Placebo groups’ rats exhibited a large number of TUNEL-positive and caspase-3 positive cells in the CA1/CA3 region(P〈0.01,n=5).(2)EA pretreatment at Baihui and Dazhui acupoints decreased the total number of TUNEL-positive and caspase-3 positive cells in CA1/CA3 region of hippocampus in comparison with Pilo and Placebo groups(P〈0.05,n=5).Conclusion:EA pretreatment at Dazhui and Baihui acupoints suppressed the SE induced hippocampal neuron apoptosis in rats.
Keywords:epilepsy  apoptosis  electroacupuncture  satus epilepticus  cognitive impairment
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