| 灯盏花提取物HPLC指纹图谱研究 |
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| 引用本文： | 孙云峰,吴品昌,赵龙山,田宝成,张平.灯盏花提取物HPLC指纹图谱研究[J].辽宁中医学院学报,2011(1):188-189. |
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| 作者姓名： | 孙云峰 吴品昌 赵龙山 田宝成 张平 |
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| 作者单位： | [1]辽宁中医药大学,辽宁沈阳110032 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]北京诺和众生国际生物科技有限公司,北京100036 |
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| 摘 要： | 目的：用高效液相色谱法建立灯盏花乙醇提取物的指纹图谱分析方法。方法：采用Waters Symmetry ShieldTMR P18色谱柱（5μm,4.6mm×250mm）;甲醇-0.4%磷酸水为流动相,线性梯度洗脱,检测波长为335nm。结果：共有22个共有峰。结论：此方法准确、重复性好,为有效地控制灯盏花药材提取物的质量提供了科学的依据。
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| 关 键 词： | 灯盏花 提取物 指纹图谱 HPLC |
Fingerprint of Herba Erigerontis Extracts by HPLC |
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| SUN Yun-feng,WU Pin-chang,ZHAO Long-shan,TIAN Bao-cheng,ZHANG Ping.Fingerprint of Herba Erigerontis Extracts by HPLC[J].Journal of Liaoning College of Traditional Chinese Medicine,2011(1):188-189. |
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| Authors: | SUN Yun-feng WU Pin-chang ZHAO Long-shan TIAN Bao-cheng ZHANG Ping |
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| Institution: | 1.Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China;2.Shenyang Pharmaceutical University,Shenyang 110016,Liaoning,China;3.The Biotechnology Ltd.CO.of Nuohezhongsheng in Beijing,Beijing 100036,China) |
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| Abstract: | Objective：To establish the HPLC-FPC of Herba Erigerontis extracts.Methods：Based on Waters Symmetry ShieldTMRP18（5μm,4.6mm×250mm）column.,methanol-0.4%phosphate acid was used as the mobile phase,and gradient elution program was applied.The detection wavelength was at 335nm.Results：22 peaks were gained on HPLC fingerprint.Conclusion：This method is simple and accurate with a good reproducibility and can be used as a quality control method for Herba Erigerontis extracts. |
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| Keywords: | herba erigerontis extracts fingerprint chromatogram HPLC |
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