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生长调节剂对款冬花产量和质量的影响
引用本文:颜永刚,刘毅.生长调节剂对款冬花产量和质量的影响[J].辽宁中医学院学报,2011(1):64-66.
作者姓名:颜永刚  刘毅
作者单位:[1]陕西中医学院药学院,陕西咸阳712046 [2]重庆邮电大学,四川重庆400065
基金项目:重庆市教委资助项目(KJ080506)
摘    要:目的:研究生长调节剂对款冬花的产量与质量的影响。方法:用不同激素处理,研究激素对产量的影响,用SPSS9.0软件处理分析;用HPLC法测定不同激素处理的款冬花的有效成分芦丁的含量。采用HPLC法,色谱柱迪马C18柱,250mm×4.6mm×0.5μm;流动相:甲醇-水(0.025moL·mL-1):磷酸(40:60);流速:1mL·min-1;波长:360nm,柱温:25℃。芦丁在0.04008~0.4008μg范围内线性关系良好(R2=0.9999),平均回收率为99.4%,(RSD=2.14%)。结果:国光比九、矮壮素能显著提高款冬花的粒重(P〈0.05),矮壮素、云大120、国光比九、植物基因活化剂能显著提高款冬花产量(P〈0.05),赤霉素能减少款冬花产量。另外,26个激素处理的款冬花样品的芦丁含量无显著性变化。结论:生长调节剂(赤霉素除外)能提高款冬花的产量,对其有效成分的含量的影响无显著差异。

关 键 词:款冬花  生长调节剂  HPLC  芦丁

Growth Conditioner to Funds Coltsfoot Output and Quality Influence
YAN Yong-gang,LIU Yi.Growth Conditioner to Funds Coltsfoot Output and Quality Influence[J].Journal of Liaoning College of Traditional Chinese Medicine,2011(1):64-66.
Authors:YAN Yong-gang  LIU Yi
Institution:1.Shaanxi College of Traditional Chinese Medicine,Xianyang 712046,Shaanxi,China;2.Chongqing Posts and Telecommunications University,Chongqing 400065,Sichuan,China)
Abstract:Objective:Research growth conditioner to funds coltsfoot's output and quality influence.Methods:With different hormone processing,the research hormone to the output the influence,processes the analysis with the SPSS9.0 software;Determines different hormone processing with the HPLC law the funds coltsfoot's effective component grass cutter's content.Uses the HPLC law,the chromatographic column enlightens the horse C18 column,250mm×4.6mm×0.5μm;Flowing:Methyl alcohol-water(0.025moL·mL-1):Phosphoric acid(40:60);Speed of flow:1mL·min-1;Wave length:360nm.Column warm:25℃;Grass cutter in 0.04008~0.4008μg scope inside connection sexual relationship good(R2=0.9999),the average returns-ratio is 99.4%(RSD=2.14%).Results:The national glory compared to nine,the cycocel can obviously enhance the funds coltsfoot the grain heavily(P0.05),the cycocel,the cloud big 120,the national glory compared to nine,the plant gene activator can obviously enhance the funds coltsfoot output(P0.05),the gibberelin can reduce the funds coltsfoot output.Moreover,26 hormone processing funds coltsfoot sample grass cutter content non-significance change.Conclusion:The growth conditioner(gibberelin exception)can enhance the funds coltsfoot the output,to its effective component content influence not remarkable difference.
Keywords:funds coltsfoot  growth conditioner  HPLC  grass cutter
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