| 河南省结核病分子流行病学调查研究 |
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| 引用本文： | 祖燕,张国龙,闫国蕊,李为民. 河南省结核病分子流行病学调查研究[J]. 医药论坛杂志, 2006, 27(1): 29-31 |
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| 作者姓名： | 祖燕 张国龙 闫国蕊 李为民 |
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| 作者单位： | 河南省卫生监督所,郑州市,450003;河南省胸科医院,郑州市,450003;河南省疾病预防控制中心,郑州市,450003;北京结核病胸部肿瘤研究所,北京市,101149 |
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| 摘 要： | 目的探讨河南省结核分支杆菌的分子流行病学分布特征。方法样本的获得采用全省范围等比例分层整群随机抽样;构建以IS6110 RFLP DNA指纹方法和以DR为基础的Spoligotyp ing DNA指纹方法;采用Gel compare 4.1软件对DNA指纹图谱进行聚类(c luster)分析。结果共获得186株可进行IS6110 RFLP DNA指纹分析的结核分支杆菌;其中59.14%(110/186)用Spoligotyp ing DNA指纹方法鉴定为“北京基因型”菌株。结论北京基因型结核分支杆菌在河南省呈较高水平的流行。
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| 关 键 词： | 分支杆菌 分子流行病学 Spoligotyping 聚类分析 |
| 文章编号： | 1672-3422(2006)01-0029-03 |
| 收稿时间： | 2005-09-09 |
| 修稿时间： | 2005-09-09 |
Molecular Epidemiological Survey of Mycobacterium Tuberculosis in Henan Province |
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| ZU Yan, ZHANG Guolong, YAN Guorui, et al. Molecular Epidemiological Survey of Mycobacterium Tuberculosis in Henan Province[J]. Journal of Medical Forum, 2006, 27(1): 29-31 |
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| Authors: | ZU Yan ZHANG Guolong YAN Guorui et al |
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| Affiliation: | Henan Institute of Sanitation Supervision, Zhengzhou 450003, China |
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| Abstract: | Objective To explore molecular epidemiological distribution characteristics of mycobacterium tuberculosis in Henan province.Methods The M.tuberculosis strains were gained by multi-stratified grouping random sampling method from the tuberculosis epidemiology survey in Henan and analyzed by IS6110-based RFLP DNA and DR-based Spoligotyping DNA fingerprinting. These fingerprinting patterns were clustered by Gel compare 4.1 software.Results 186 M.tuberculosis strains obtained from this survey can be conducted IS 6110 RFLP DNA fingerprinting analysis,among which 59.14%(110/186) were determined to be "Beijing Genotype" M.Tuberculosis strain by spoligotyping DNA fingerprinting analysis.Conclusion M.tuberculosis strains of Beijing Genotype are prevailing in Henan at present. |
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| Keywords: | Spoligotyping |
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