HPLC双波长梯度洗脱同时测定通塞脉微丸中绿原酸、阿魏酸、甘草苷的含量

 目的研究同时测定通塞脉微丸中绿原酸、阿魏酸及甘草苷含量的方法。方法KromasilC₁₈色谱柱,乙腈-2%冰醋酸为流动相,梯度洗脱,检测波长λ₁=324nm,λ₂=276nm。结果绿原酸、阿魏酸、甘草苷分别在0.2084～3.334、0.05035～0.8056,0.2315～3.704μg内有良好的线性关系,r>0.9999;平均加样回收率分别为99.07%,100.40%,98.13%,相应的RSD分别为0.73%,1.32%,0.97%(n=6)。结论本法操作简便,结果可靠,重现性好,可作为产品质量控制的方法。

Determination of Chlorogenic Acid and Ferulic Acid and Liquiriitin in Tong Sai Mai Tinypil by Dual Wavelength High-Performance Liquid Chromatography with Gradient Elution

OBJECTIVE To establish a method for the determination of chlorogenic acid and ferulic acid and liquiriitin in Tong Sai Mai tinypil.METHODS The separation was performed on a Kromasil C₁₈ column with the mobile phase consisting of acetonitrile-2% acetic acid for gradient elution.Chlorogenic acid,ferulic acid and liquiriitin were determined by dual wavelength of 324 nm(λ₁)and 276 nm(λ₂).RESULTS The linearities were in the ranges of 0.208 4-3.334 μg for chlorogenic acid,0.050 35-0.805 6μg for ferulic acid,and 0.231 5-3.704 μg for liquiriitin,respectively.The average recoveries was 99.07%(RSD=0.73% n=6),100.40%(RSD=1.32% n=6),98.13%(RSD=0.97% n=6),respectively.CONCLUSION This method is convenient,rapid and accurate.It can be used for quality control in production of Tong Sai Mai tinypill.