| DNA条形码技术在陕西省鼠疫疫区宿主动物鉴定中的应用 |
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| 引用本文： | 安翠红,孙养信,陈宝宝,范锁平,霍丽霞,佘建军,吕文. DNA条形码技术在陕西省鼠疫疫区宿主动物鉴定中的应用[J]. 中华流行病学杂志, 2014, 35(9): 1042-1045 |
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| 作者姓名： | 安翠红 孙养信 陈宝宝 范锁平 霍丽霞 佘建军 吕文 |
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| 作者单位： | 陕西省疾病预防控制中心鼠布生防科,西安,710054 |
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| 基金项目： | 陕西省科学技术研究发展计划，a grant from the Science and Technology Research and Development Program of Shaanxi Province |
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| 摘 要： | 目的 探讨DNA条形码技术在鼠疫疫区宿主动物鉴定中的应用,并建立陕西省鼠疫疫区宿主动物DNA条形码数据库.方法 运用DNA条形码技术检测陕西省定边县鼠疫疫区3目6科12属14种139只宿主动物以及7个鼠种8个不同部位的62份残体标本线粒体细胞色素C氧化酶Ⅰ亚基基因序列.结果 种内遗传距离≤2％,种间遗传距离为8.9％～ 15.1％;邻接法系统树能区分14个不同物种的类群;采集的残体标本能检测到目的基因并用于鉴定;研究中还纠正了既往定边县鼠疫疫区黄鼠命名.结论 DNA条形码技术能弥补形态学鉴定的不足,可用于鼠疫疫区宿主动物及残体标本的分类鉴定.
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| 关 键 词： | 鼠疫疫区 DNA条形码 基因,线粒体细胞色素C氧化酶Ⅰ亚基 鼠种鉴定 残体鉴定 |
| 收稿时间： | 2014-02-21 |
Identification on host animals for plague by DNA barcoding technology in Shaanxi province |
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| An Cuihong,Sun Yangxin,Chen Baobao,Fan Suoping,Huo Lixi,She Jianjun and Lyu Wen. Identification on host animals for plague by DNA barcoding technology in Shaanxi province[J]. Chinese Journal of Epidemiology, 2014, 35(9): 1042-1045 |
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| Authors: | An Cuihong Sun Yangxin Chen Baobao Fan Suoping Huo Lixi She Jianjun Lyu Wen |
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| Affiliation: | Department for Plague, Brucellosis and Etiologic Biological Vector Control, Shaanxi Provincial Center for Disease Control and Prevention, Xi''an 710054, China;Department for Plague, Brucellosis and Etiologic Biological Vector Control, Shaanxi Provincial Center for Disease Control and Prevention, Xi''an 710054, China;Department for Plague, Brucellosis and Etiologic Biological Vector Control, Shaanxi Provincial Center for Disease Control and Prevention, Xi''an 710054, China;Department for Plague, Brucellosis and Etiologic Biological Vector Control, Shaanxi Provincial Center for Disease Control and Prevention, Xi''an 710054, China;Department for Plague, Brucellosis and Etiologic Biological Vector Control, Shaanxi Provincial Center for Disease Control and Prevention, Xi''an 710054, China;Department for Plague, Brucellosis and Etiologic Biological Vector Control, Shaanxi Provincial Center for Disease Control and Prevention, Xi''an 710054, China;Department for Plague, Brucellosis and Etiologic Biological Vector Control, Shaanxi Provincial Center for Disease Control and Prevention, Xi''an 710054, China |
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| Abstract: | |
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| Keywords: | Plague area DNA barcoding Mitochondrial cytochrome C oxidase subunit Ⅰ gene Species identification Residues identification |
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