| 紫外分光光度法测定荡气颗粒中大黄结合蒽醌的含量 |
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| 引用本文： | 王丽君,刘永,田军,杨士友.紫外分光光度法测定荡气颗粒中大黄结合蒽醌的含量[J].安徽医药,2012,16(6):761-763. |
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| 作者姓名： | 王丽君 刘永 田军 杨士友 |
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| 作者单位： | 安徽中医学院药学院,安徽,合肥,230031;安徽省药物研究所;安徽省中药研究与开发重点实验室,安徽,合肥,230022;安徽省食品药品审评认证中心,安徽,合肥,230051 |
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| 摘 要： | 目的建立荡气颗粒中大黄结合蒽醌含量紫外分光光度测定方法。方法先采用三氯甲烷超声振荡提取除去游离蒽醌,残渣用盐酸水解得到结合蒽醌水解物;利用大黄蒽醌类化合物能与醋酸镁反应显色的原理,以显色剂为空白,测定样品溶液在517 nm波长处得吸收度。结果对照品大黄素含量在2.1～16.8 mg·L-1范围内,浓度与吸光度之间线性关系良好(r=0.9999);样品的平均回收率为98.6%,RSD%为1.2%(n=6)。结论紫外分光光度法适用于荡气颗粒中结合蒽醌的含量测定。
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| 关 键 词： | 紫外分光光度法 荡气颗粒 结合蒽醌 含量测定 |
Determination of combined anthraquinones in Dangqi Granule by direct ultraviolet spectrophotometry |
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| Institution: | WANG Li-jun, LIU Yong ,TIAN Jun , et al (1. College of Pharmacy,Anhui College of Traditional Chinese Medicine, Hefei ,Anhui 230031, China;2. Anhui Institute of Materia Medica ;3. Anhui Province Key Laboratory of R＆D of Chinese Medicine ,Hefei ,Anhui 230022, China) |
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| Abstract: | Objective To establish a method for the determination of the combined anthraquinones of Dangqi Granule. Methods Tri- chloromethane was used to abstract free anthraquinone by ultrasonic vibration, hydrochloric acid was used to hydrolyze after drying. Ac- cording to the theory that colouring reaction will occur while anthraquinones in rhubarb react upon magnesium acetate, the absorption val- ue of the sample at 517 nm was measured to caculate content directly. Results The calibration curve of the absorbency was linear with the concentration of emodin in the range of 2.1 - 16.8 mg · L^- 1, （ r = 0.999 9）. The average recovery of emodin was 98.6%, and RSD was 1.2% （n = 6）. Conclusion The method can be applied to determine combined anthraquinones in Dangqi Granule. |
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| Keywords: | UV speetrophotometry Dangqi Granule combined anthraquinones content |
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