黄芪丹参茯苓龟板合剂对大鼠肾间质纤维化的抑制作用

目的 探讨黄芪丹参茯苓龟板合剂对肾间质纤维化进展过程的干预作用及其机制.方法 通过单侧输尿管梗阻法建立肾间质纤维化的大鼠模型,实验分为假手术(Sham) 组、单侧输尿管梗阻 (UUO) 组、血管紧张素转换酶抑制剂(ACEI) 组及黄芪丹参茯苓龟板合剂(中药)组,术后第14天取大鼠肾组织进行常规HE、Masson染色及C...

An experimental study on traditional Chinese medicine for prevention of the renal tubulointerstitial fibrosis

Objective To investigate the interventional effect of Chinese herbs on renal tubulointerstitial fibrosis after unilateral urethral obstruction(UUO) in rats.Methods The experimental rats were randomly divided into four groups:sham-operated group for control(Sham group),unilateral ureteral obstruction group(UUO group),angiotensin converting enzyme inhibitor treatment group(ACEI group) and Chinese herbs group.The kidney tissues were collected from each group on 14d after operation.Hematoxylin-eosin stain and Masson stain were used to measure tubulointerstitial fibrosis and immunohistochemical method was performed to detect CD68 and α-smooth muscle actin.Results(1)The degree of renal tubulointerstitial fibrosis,and the expression of CD68,α-SMA were reduced in ACEI group and Chinese herbs group compared with UUO group(P<0.05).(2)The degree of renal tubulointerstitial fibrosis,the positive rate of CD68 and α-SMA was lower in Chinese herbs group compared with ACEI group(P<0.05).Conclusion Chinese herbs can prevent the renal interstitial fibrosis after unilateral urethral obstruction in rats,through inhibiting the infiltration of mononuclear macrophage in mesenchymal and transdifferentiation of interstitial cells.