卵巢上皮性肿瘤中claudin3、claudin4的表达及其意义

目的探讨claudin3、4与卵巢癌的发生、发展关系。方法使用免疫组化及原位杂交方法检测卵巢上皮、浆液性及黏液性囊腺瘤、交界性囊腺瘤、原发性卵巢癌、浆液性囊腺癌的远处转移灶及卵巢转移性癌的石蜡包埋组织中claudin3、4蛋白及claudin4-mRNA的表达情况。结果 claudin3、4蛋白及claudin4-mRNA在卵巢癌中表达强于交界性肿瘤,交界性肿瘤强于良性肿瘤,但是交界性浆液性肿瘤和良性肿瘤表达无明显差异;在卵巢癌的转移灶中表达程度类似于原发灶;在转移至卵巢的癌中的表达明显弱于卵巢原发癌。结论 claudin3、4表达增强与原发性卵巢癌的发生、发展、转移密切相关;浆液性囊腺癌可以有良性肿瘤直接恶变,也可以由交界性肿瘤发展而来,但不经历良性-交界性转变过程;黏液性囊腺癌则不仅可以由良性肿瘤直接恶变,也可以经历良性-交界性-恶性发展过程。

Expression and singificance of claudin3 and claudin4 in epithelial tumor of ovary

Objective To explore the effects of claudin3,4 on genesis and development of ovary cancinoma.Methods Immunohistochemical and In situ hybridization（ISH） methods were employed to detect the expression of claudin3,4 proteins and claudin4-mRNA in normal epithelium,benign cystadenoma,cystadenoma of borderline,all kinds of ovary carcinoma,metastasis of serous cystadenocarcinoma,metastatic carcinoma to ovary.Results Claudin3,4 protein and mRNA hardly expressed in normal ovarian epithelium and epithelial tumor of benign,and were obviously weaker than in tumor of borderline,but there were no distinction between borderline serous tumor and serous tumor.The expression of claudin3,4 in borderline tumors were obviously weaker than in all kinds of ovarian carcinoma.The expression in metastasis was similar to that at ovarian.The expression in metastasis at ovary of other tumor was obviously weaker than in ovarian carcinoma.Conclusion The enhancement of expression of claudin3,4 are closely correlated with the genesis,developement and metastasis of ovarian carcinoma.Serous carcinoma can transform from benign tumor or from serous borderline tumor,but benign tumor can＇t transform to borderline tumor.The ovarian mucinous carcinoma can transform from benign and borderline tumor,or change from benign to borderline then to carcinoma.