| HPLC法测定复方五味子补肾颗粒中的五味子醇甲的含量 |
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| 引用本文： | 万立夏,张树强. HPLC法测定复方五味子补肾颗粒中的五味子醇甲的含量[J]. 安徽医药, 2012, 16(8): 1090-1091 |
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| 作者姓名： | 万立夏 张树强 |
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| 作者单位： | 安徽省五河县中医院,安徽,蚌埠,233300;安徽省蚌埠市食品药品监督管理局,安徽,蚌埠,233000 |
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| 摘 要： | 目的建立复方五味子补肾颗粒中五味子醇甲的含量测定方法。方法采用高效液相色谱法测定复方五味子补肾颗粒中五味子醇甲的含量。Welch Materials C18色谱柱(4.6 mm×250 mm,5μm);甲醇-水(70∶30)为流动相;流速:1.0 ml.min-1;柱温25℃。结果在高效液相色谱法图中,五味子醇甲与其它组份分离良好,线性范围为0.024 4～0.244 g.L-1,其回归方程为:Y=39 648.426X+51.18,r=0.999 9(n=6),线性良好为0.024 4～0.244 g.L-1,平均加样回收率为99.46%(RSD=0.54%)。结论该方法可以很好的用于控制复方五味子补肾颗粒的质量。
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| 关 键 词： | 复方五味子补肾颗粒 五味子醇甲 HPLC 含量测定 |
Determination of schizandrol A in fufang wuweizi bushen granules by HPLC |
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| WAN Li-xia , ZHANG Shu-qiang. Determination of schizandrol A in fufang wuweizi bushen granules by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2012, 16(8): 1090-1091 |
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| Authors: | WAN Li-xia ZHANG Shu-qiang |
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| Affiliation: | (Wuhe Traditional Chinese Medicine Hospital,Bengbu,Anhui 233300; Bengbu Food & Drug Administration,Anhui 233000) |
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| Abstract: | Objective To establish a method for determination of Schisandrin in fufang wuweizi bushen granules.Methods The content of Schisandrin was determined by HPLC.Welch Materials C18 column(4.6 mm×250 mm,5 μm)with mobile phase of methanol-water(70-30) was used with a flow rate of 1.0 ml·min-1.Results The regression equation was Y=39 648.426X+51.18 and coefficient correlation was 0.999 9(n=6).The linear range of Schisandrin was from 0.024 4 to 0.244 g·L-1 and recovery rate was 99.46%,and RSD was 0.54%.Conclusion This method can be used to control the quality of Schisandrin in Fufang Wuweizi Bushen granules better. |
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| Keywords: | fufang wuweizi bushen granules Schizandrol A HPLC content determination |
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