| 紫外吸收碘量法快速测定体液维生素C |
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| 引用本文： | 朱小云,李生兵,郭睿,廖飞,王咏梅,左渝萍. 紫外吸收碘量法快速测定体液维生素C[J]. 重庆医科大学学报, 2004, 29(6): 841-842,848 |
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| 作者姓名： | 朱小云 李生兵 郭睿 廖飞 王咏梅 左渝萍 |
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| 作者单位： | 重庆医科大学生物化学教研室,重庆,400016;重庆医科大学2001级七年本科学生,400016 |
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| 基金项目： | 重庆市教委首批中青年骨干教师资助计划。 |
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| 摘 要： | 目的 :考察用紫外吸收碘量法快速测定体液维生素C的可行性。方法 :用碘乙酸封闭巯基 ,三氯乙酸沉淀蛋白质 ,二氯甲烷抽提除去干扰色素。测定处理后体液样品与碘反应 30s前后 35 3nm吸收变化。结果 :测定反应前或后的光吸收变异<5 % ,反应前后光吸收变化同体系VC含量成正比 ,响应曲线斜率与三碘复合物的消光系数接近 ,样品中VC回收率 >90 % ,可测定反应体系 5～ 30 μmol/L的VC。 结论 :本方法有望用于体液VC的快速测定。
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| 关 键 词： | 维生素C 碘量法 紫外吸收检测 |
| 文章编号： | 0253-3626(2004)06-0841-02 |
Fast assay of vitamin C in body fluids by iodometry with UV absorbance detection |
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| ZHU Xiaoyun,et al. Fast assay of vitamin C in body fluids by iodometry with UV absorbance detection[J]. Journal of Chongqing Medical University, 2004, 29(6): 841-842,848 |
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| Authors: | ZHU Xiaoyun et al |
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| Abstract: | Objective:To investigate the feasibility of fast assay of vitamin C (VC) in body fluids by iodometry with UV absorbance detection.Methods:The sample was pretreated with iodoacetic acid to block sulfhydryl group.Protein was precipitated by trichloroacetic acid,and chromogen was extracted with chloromethylene.The change of absorbance after 30 s reaction of the pretreated sample with iodine was measured.Results:The assay of absorbance before and after 30 s reaction of the pretreated sample with iodine showed the variation coefficient within 5%,and the recovery of VC was above 90%.There was linear response of the absorbance change to the amount of VC from body fluids with slope consistent to that for iodine.Conclusion:This method may be desirable for fast assay of VC in body fluids. |
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| Keywords: | Vitamin C Iodometry UV absorbance detection |
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