喉鳞状细胞癌及转移淋巴组织中Cyclin E的表达

目的：定量检测细胞周期素E（Cyclin E）在喉鳞状细胞癌及颈部淋巴组织中的表达，探讨其在喉癌转移中的可能作用。方法：采用激光共聚焦显微镜、多参数流式细胞技术对手术切除的21例喉鳞状细胞癌（7例伴颈淋巴结转移，14例不伴颈淋巴结转移）组织标本中Cyclin E的表达含量进行检测分析。结果：Cyclin E在喉鳞状细胞癌、阳性淋巴结、阴性淋巴结中均有不同程度的表达，喉鳞状细胞癌伴颈淋巴结转移平均荧光强度为78.12士6.54，略高于不伴颈淋巴结转移者（74.93士8．82）（P〉0．05）；阳性淋巴结平均荧光强度为93．65士7．48，明显高于阴性淋巴结（30．65士7．48）（P〈0．01），且高于喉鳞状细胞癌伴颈淋巴结转移和不伴颈淋巴结转移者（P〈0．05）。结论：Cyclin E的异常表达是喉癌发生、发展过程中一个重要的分子事件；Cyclin E的检测可望成为临床评估喉癌淋巴结转移的指标之一。

Detection of Cyclin E in lymph node metastases of laryngeal squamous cell carcinomas by laser scanning confocal microscopy

OBJECTIVE: To study the expression of Cyclin E proteins in carcinogenesis and development of the metastasis of laryngeal squmous cell carcinomas (LSCC). METHOD: The expression of Cyclin E protein were detected by laser scanning confocal microscopy (LSCM) and multiparameter flow cytometry (FCM) after immunofluorescence staining method in twenty-one surgically resected primary LSCC (7 LSCC with cervical lymph node metastasis, 14 LSCC without cervical lymph node metastasis). RESULT: The fluoresence intensity was 78.12 +/- 6.54 in LSCC with cervical lymph node metastasis and 74.93 +/- 8. 82 in LSCC without cervical lymph node metastasis (P > 0. 05); The fluoresence intensity in cervical lymph node metastasis was 93.65 +/- 7.48, it's more highter than that in cervical lymph node negative metastasis (P < 0.01) and both LSCC with cervical lymph node metastasis and without cervical lymph node metastasis (P < 0.05). CONCLUSION: It is suggested that Cyclin E proteins may play an important role in the carcinogenesis and development of the lymph node metastases of LSCC. Detection of Cyclin E maybe becomes an indication to evaluating the lymph node metastases of LSCC.