罗格列酮对糖尿病大鼠肾组织nephrin表达的影响

目的:探讨糖尿病大鼠肾组织nephrin的表达以及罗格列酮干预后对其表达的影响。方法:链脲佐菌素(STZ)诱导糖尿病大鼠模型,将糖尿病大鼠随机分为糖尿病非干预组(DM组)和罗格列酮干预组(DR组),并以正常组(NC组)作对照。干预8周后,观察各组大鼠尿蛋白、血糖、血脂、血尿素氮(BUN)、血肌酐(Cr)变化;用免疫组化及实时荧光定量PCR检测肾组织nephrin蛋白及mRNA的表达。结果:①DM组大鼠尿蛋白、血BUN,Cr较NC组明显升高,肾组织nephrin蛋白及mRNA表达降低;与DM组相比,DR组大鼠尿蛋白、血BUN,Cr降低,nephrin蛋白及mR-NA表达上调。②DM组血糖、血脂与NC组相比,差异有统计学意义,与DR组比较差异无统计学意义。结论:罗格列酮可能通过上调糖尿病大鼠肾组织nephrin表达,发挥不依赖糖脂代谢的直接肾保护作用。

Effect of rosiglitazone on the expression of nephrin in the kidney of diabetic rats

Objective: To investigate the effect of rosiglitazone on the expression of nephrin in the kidney of diabetic rats.Methods: Diabetic model was established by a single injection of streptozotocin(STZ),and diabetic rats were randomly divided in to 2 groups: untreated diabetes mellitus group(DM group) and rosiglitazone-treated diabetes mellitus group(DR group).In addition,the normal rats served as a normal control group(NC group).Eight weeks later,the excretion of urine protein,blood glucose,serum lipid profile,serum urea nitrogen and serum creatinine were detected.The expression of nephrin was detected by immunohistochemistry and real-time Q-PCR. Results: Compared with NC group,the excretion of urine protein,serum urea nitrogen and serum creatinine in DM group were significantly increased,and the expression of nephrin was significantly down-regulated.Compared with DM group,the excretion of urine protein,serum urea nitrogen and serum creatinine in DR group were significantly decreased,and the expression of nephrin was significantly up-regulated.Blood glucose and serum lipid profile were significantly changed in DM group compared with NC group,while they had no significant difference between DM group and DR group. Conclusion: Rosiglitazone may up-regulate the expression of nephrin in the kidney of diabetic rats to produce direct nephroprotective effects independent of glucose and lipid metabolism.