| BALB/c裸鼠脾内移植人源性永生化肝细胞HepLL的生物学特性 |
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| 引用本文： | 章益民,李兰娟,李君,盛国平,喻成波,俞海英. BALB/c裸鼠脾内移植人源性永生化肝细胞HepLL的生物学特性[J]. 浙江医学, 2008, 30(11) |
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| 作者姓名： | 章益民 李兰娟 李君 盛国平 喻成波 俞海英 |
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| 作者单位： | 浙江大学医学院附属第一医院传染病诊治国家重点实验室,杭州,310003 |
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| 基金项目： | 国家高技术研究发展计划(863计划) ， 国家自然科学基金重点项目 |
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| 摘 要： | 目的研究人源性肝细胞HepLL经脾内途径移植后在体内的生物学特性。方法将人源性肝细胞HepLL移植入切除肝左叶和未切除肝左叶的BALB/c裸鼠脾内,通过病理和免疫组化方法检测其生物学特性。结果新建的人源性肝细胞移植入BALB/c裸鼠脾脏后可长期成团存活、增殖并表达肝细胞特异性标记物HEP,且有糖原的堆积,同时始终未检测到甲胎蛋白的表达。至移植后第30天,脾脏内移植的HepLL细胞切除左肝组比未切左肝组增生活跃。移植入脾脏的人源性肝细胞可向宿主肝脏移行并进入肝实质,同时可长期在宿主肝脏内增殖。结论移植入脾脏的人源性肝细胞HepLL可保持其原有的生物学特性,并能移行、整合入肝脏;肝左叶切除造成的肝细胞再生微环境有利于移植的人源性肝细胞HepLL的增殖。
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| 关 键 词： | 裸鼠 人源性永生化肝细胞 肝细胞移植 |
Biological characteristics of immortalized human hepatocytes(HepLL)after transplantation in spleen of BALB/c nude mice |
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| ZHANG Yimin,LI Lanjuan,LI Jun,et al.. Biological characteristics of immortalized human hepatocytes(HepLL)after transplantation in spleen of BALB/c nude mice[J]. Zhejiang Medical Journal, 2008, 30(11) |
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| Authors: | ZHANG Yimin LI Lanjuan LI Jun et al. |
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| Affiliation: | ZHANG Yimin,LI Lanjuan,LI Jun,et al.State Key Laboratory for Diagnosis , Treatment of Infectious Diseases,the First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China |
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| Abstract: | Objective To investigate the biological characteristics of immortalized human hepatocyte(HepLL)after transplantation in the spleen of BALB/c nude mice.Methods The HepLL cells were transplanted into the spleen in BALB/c nude mouse with left hepatic lobe resected or reserved.The biological characteristics of HepLL cells in spleen were examined by pathology and immunohistochemistry methods.Results The HepLL cells survived in cluster for a long period after transplantation.The cells maintained ability of prolif... |
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| Keywords: | Nude mice Immortalized human hepatocytes Hepatocyte transplantation |
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