VEGF、GLUT1在肺癌中的表达及与血管生成的关系

目的 探讨血管内皮生长因子(VEGF)与葡萄糖转运蛋白1(GLUT1)在肺癌中的表达及与肿瘤血管生成之间的关系.方法 通过免疫组化Power Vision Tm二步法检测54例肺癌组织及10例正常组织中VEGF、GLUTI的表达,应用CD34单克隆抗体标记,测定微血管密度(MVD).结果 79.6%肺癌中VEGF表达为阳性,61.1%肺癌中GLUT1表达为阳性,VEGF、GLUT1的表达及MVD的评分均与肺癌的TNM分期、组织学分级、淋巴结转移显著相关.VEGF、GLUT1的表达与MVD显著相关.结论VEGF、GLUT1在肺癌组织中呈过表达;肺癌中VEGF和GLUT1的表达与MVD密切相关,VEGF和GLUT1可能参与血管生成,而肿瘤血管生成则是致癌机制之一.

The Expression of VEGF and GLUT1 in Lung Cancer and Their Influence to Tumor Angiogenesis

Objective To study the expression of VEGF (vascular endothelial growth factor)and GLUT1 (glucose transpoter-1)in human lung cancer and their influence to tumor angiogenesis. Methods Ar-chival primary lung cancer(n=54) and normal lung tissue(n'=10) were analyzed for VEGF, GLUT1 expres-sion by Power VisionTM Two-Step immunohistochemistry using specific monoclonal antibodies. We also examined the MVD (microvessel denisty), using CD34 staining. Results 79.6% of lung carcinomas showed positive ex-pression of VEGF,61.1% of lung carcinomas showed positive expression of GLUT1. Both VEGF,GLUT1 expres-sion level and the score of MVD were correlated with TNM stage, lymphnode metastasis, histological grade. VEGF and GLUT1 expression level were correlated with the score of MVD. Conclusion Compared with normal tissue,VEGF and GLUT1 were overexpressed in tumor specimens. Both VEGF and GLUT1 were significantly correlated with MVD. Tumor angiogenesis may be related to the carcinogeneses and tumor progression of VEGF and GLUT1.