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| Changes of biological functions of dipeptide transporter （PepT1） and hormonal regulation in severe scald rats | |
| 作者姓名： | Sun BW Zhao XC Wang GJ Li N Li JS |
| 作者单位： | Bing-Wei Sun(Department of GeneralSurgery, School of Medicine, Nanjing University, Nanjing 210093,Jiangsu Province, China;Research Institute of General Hospital, Chinese PLA General Hospital of Nanjing Military Command, Nanjing 210002,Jiangsu Province,China);Xiao-Chen Zhao,Guang-ji WANG(Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China);Ning Li(Department of GeneralSurgery, School of Medicine, Nanjing University, Nanjing 210093,Jiangsu Province, China;Research Institute of General Hospital, Chinese PLA General Hospital of Nanjing Military Command, Nanjing 210009, Jiangsu Province, China);Jie-Shou Li(Department of GeneralSurgery, School of Medicine, Nanjing University, Nanjing 210093,Jiangsu Province, China;Research Institute of General Hospital, Chinese PLA General Hospital of Nanjing Military Command, Nanjing 21000) |
| 摘 要： | AIM: To determine the regulatory effects of recombinant human growth hormone (rhGH) on dipeptide transport (PepT1) in normal and severe scald rats. METHODS: Male Sprague-Dawley rats with 30 % total body surface area (TBSA) III degree scald were employed as the model. In this study rhGH was used at the dose of 2 IU.kg^1.d^-1 An everted sleeve of intestine 4 cm long obtained from mid-jejunum was securely incubated in Kreb‘s solution with radioactive dipeptide (3H-glycylsarcosine, 3H-Gly-Sar,10μCi/ml) at 37℃ for 15 min to measure the effects of uptake and transport of PepT1 of small intestinal epithelial cells in normal and severe scald rats. RESULTS: Abundant blood supply to intestine and mesentery was observed in normal and scald rats administered rhGH, while less supply of blood to intestine and mesentery was observed in rats without rhGH. Compared with controls, the transport of dipeptide in normal rats with injection of rhGH was not significantly increased (P=-0.1926), while the uptake was significantly increased (P=-0.0253). The effects of transport and uptake of PepT1 in scald rats with injection of rhGH were significantly increased (P=0.0082, 0.0391). CONCLUSION: Blood supply to intestine and mesentery of rats was increased following injection of rhGH. The effectsof uptake and transport of dipeptide transporters in small intestinal epithelial cells of rats with severe scald were markedly up-regulated by rhGH. |
| 关 键 词： | 缩二氨酸转运物 荷尔蒙 严重烫伤 动物实验 作用机制 |
| 收稿时间： | 2003 Jun 10 |
Changes of biological functions of dipeptide transporter (PepT1) and hormonal regulation in severe scald rats |
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| Sun BW,Zhao XC,Wang GJ,Li N,Li JS.Changes of biological functions of dipeptide transporter (PepT1) and hormonal regulation in severe scald rats[J].World Journal of Gastroenterology,2003,9(12):2782-2785. | |
| Authors: | Sun Bing-Wei Zhao Xiao-Chen Wang Guang-Ji Li Ning Li Jie-Shou |
| Institution: | 1. Department of GeneralSurgery, School of Medicine, Nanjing University, Nanjing 210093,Jiangsu Province, China;Research Institute of General Hospital, Chinese PLA General Hospital of Nanjing Military Command, Nanjing 210002,Jiangsu Province,China 2. Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China 3. Department of GeneralSurgery, School of Medicine, Nanjing University, Nanjing 210093,Jiangsu Province, China;Research Institute of General Hospital, Chinese PLA General Hospital of Nanjing Military Command, Nanjing 210009, Jiangsu Province, China 4. Department of GeneralSurgery, School of Medicine, Nanjing University, Nanjing 210093,Jiangsu Province, China;Research Institute of General Hospital, Chinese PLA General Hospital of Nanjing Military Command, Nanjing 21000 |
| Abstract: | AIM: To determine the regulatory effects of recombinant human growth hormone (rhGH) on dipeptide transport (PepT1) in normal and severe scald rats. METHODS: Male Sprague-Dawley rats with 30% total body surface area (TBSA) III degree scald were employed as the model. In this study rhGH was used at the dose of 2 IU/kg(-1)d(-1). An everted sleeve of intestine 4 cm long obtained from mid-jejunum was securely incubated in Kreb's solution with radioactive dipeptide (3H-glycylsarcosine, 3H-Gly-Sar, 10 microCi/ml) at 37 degrees C for 15 min to measure the effects of uptake and transport of PepT1 of small intestinal epithelial cells in normal and severe scald rats. RESULTS: Abundant blood supply to intestine and mesentery was observed in normal and scald rats administered rhGH, while less supply of blood to intestine and mesentery was observed in rats without rhGH. Compared with controls, the transport of dipeptide in normal rats with injection of rhGH was not significantly increased (P=0.1926), while the uptake was significantly increased (P=0.0253). The effects of transport and uptake of PepT1 in scald rats with injection of rhGH were significantly increased (P=0.0082, 0.0391). CONCLUSION: Blood supply to intestine and mesentery of rats was increased following injection of rhGH. The effects of uptake and transport of dipeptide transporters in small intestinal epithelial cells of rats with severe scald were markedly up-regulated by rhGH. |
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