中药肠安泰体外对Co26lu大肠癌细胞的血清药理学研究

目的:应用血清药理学方法检测中药肠安泰及其主要成分的药物血清对Co26 lu大肠癌细胞体外杀伤能力。方法:5组大鼠除5-Fu血清组按30mg/kg予腹腔注射,给药2h后腹主动脉采血外,其余4组均按0.6m l.kg-1.d-1分别给予肠安泰(干膏)、大黄(干膏)、藤梨根(干膏)、生理盐水灌胃,选用2种不同采血时间。一日采血:用药1h后眶静脉采血2m l;三日采血:末次用药后1h腹主动脉采血,无菌分离血清。采用MTT法观察药物血清对细胞的杀伤作用。结果:肠安泰及其单味药与5-Fu的不同浓度药物血清对Co26 lu细胞均有不同程度的体外杀伤能力,并呈剂量依赖性。结论:肠安泰能够抑制肿瘤细胞的生长及转移。

Serum Pharmacology Research on Vitro Effects of Changantai on Co26lu Colon Cancer cells

Objective: To detect the ability of medicine serum of Changantai and its major components to kill Co26lu colon cancer cells in vitro by means of serum pharmacology.Methods: There were 5 groups of rats.5-Fu 30mg/kg were injected into the abdominal cavity of the rats in 5-Fu blood serum group and abdominal aorta blood was drawn 1 hour after administration.The other four groups were intragastrically administrated with Changantai(dry paste),rhubarb(dry paste),radix actinidiae(dry paste) and NS 0.6ml/kg/d respectively and the blood was draw twice,that was,2ml orbital vein hemospasia 1 hour after administration and abdominal aorta hemospasia 1 hour after the last administration of three consecutive days with asceptic serum segregation.Ability of medicine serum to kill tumor cells was observed by means of MTT method.Results: medicine serum of 5-Fu,Changantai and its components with different concentration had different ability in vitro to kill Co26lu cells,which was concentration dependent.Conclusion: Changantai can inhibit cancer cells from growing and metastasis.