犬PRF复合BMSCs修复拔牙窝颊侧骨壁缺损的实验研究

目的:评价富血小板纤维蛋白(PRF)构建的组织工程骨,在颊侧骨壁缺损修复中的成骨作用。方法:体外将PRF作用于比格犬的骨髓间充质干细胞(BMSCs),第21天测定钙结节量;4、7、11 d时采用实时定量PCR法检测成骨相关基因。9只成年比格犬拔除上颌左右两侧的侧切牙,构建颊侧骨壁缺损的拔牙位点,并随机分为血凝块组、PRF组和组织工程骨组,在手术后即刻、6周及12周时评价各组的颊侧骨高度和拔牙窝的骨密度。结果:PRF在体外实验中能明显促进BMSCs的增殖,提高成骨分化以及钙化能力(P<0.05)。动物实验中,PRF表现出了促进早期伤口愈合的能力。在颊侧骨壁缺损的修复中,组织工程组在6周时骨高度和骨密度分别为(2.63±0.57)mm、(765.19±59.70)HU,较血凝块组[(5.65±2.91)mm、(599.08±53.88)HU]和PRF组[(4.14±1.16)mm、(644.76±65.39)HU]显著增高(P<0.05)。结论:PRF复合BMSCs构建的组织工程骨可以在早期有效地修复拔牙窝骨壁缺损。

Cooperative Effects of PRF and BMSCs in Alveolar Bone Regeneration in a Canine Model

Objective： To evaluate platelet-rich fibrin （PRF） scaffolds and BMSCs in repairing mandibular buccal bone defects at canine′s tooth sockets. Methods： Fabrication of PRF were combined with beagle′s BMSCs scaffolds in vitro, and in day 21, calcium nodules were detected. Real time PCR were performed at days 4, 7 and 11. Nine adult beagle dogs were used to extract the left and right maxillary lateral incisor in vivo forming buccal bone defects. Dogs were randomly divided into 3 groups ： blood clots group, PRF group and bone tissue engineering group. Evaluating buccal bone height in each group and the extraction socket bone density were performed at four time points： immediately after surgery, 6 week and 12 weeks later respectively. Results： In vitro, the PRF increased the BMSCs proliferation and significantly improve the ability of osteogenic differentiation and calcification （P〈0.05）. In animal experiments, PRF showed early healing wound ability. On repairing bone defects, at 6 weeks in the tissue engineering group, the bone hight [（2.63±0.57） mm] and bone mineral density [（765.19±59.70） HU] was significantly increased （P0.05）, which compared with blood clots group [（5.65±2.91） mm,（599.08±53.88） HU] and PRF group [（4.14±1.16） mm,（644.76±65.39） HU]. Conclusion： Cooperative of PRF and BMSCs can effectively repair mandible alveolar bone defects in canine model.