| 大鼠面神经损伤后神经元型一氧化氮合酶的表达变化 |
| |
| 引用本文： | 王鹏,臧晓燕,张引成. 大鼠面神经损伤后神经元型一氧化氮合酶的表达变化[J]. 口腔颌面外科杂志, 2014, 0(4): 272-275 |
| |
| 作者姓名： | 王鹏 臧晓燕 张引成 |
| |
| 作者单位： | 王鹏 (同济大学口腔医学院口腔生物医学及转化医学实验室,上海,200072)； 臧晓燕 (同济大学口腔医学院口腔生物医学及转化医学实验室,上海,200072)； 张引成 (西安交通大学口腔医学院口腔颌面外科教研室,陕西 西安,710004)； |
| |
| 摘 要： | 目的:通过观察面神经损伤后神经元型一氧化氮合酶(neuronal NOS,nNOS)在面神经核团中的表达,探讨NO在面神经损伤后的作用。方法:健康成年SD大白鼠20只,其中5只作为正常对照。其余15只为面神经损伤组,在双侧面神经下颊支制作挤压伤。术后1、2、3、4、5周各取3只损伤组大鼠作面神经核团nNOS表达的免疫组化观察。结果:正常情况下面神经元不表达nNOS。面神经损伤后,出现运动神经元变性,其神经元细胞nNOS免疫染色为阳性,并持续至损伤后第5周。结论:面神经损伤后其运动神经元的核团中会出现nNOS的表达。
|
| 关 键 词： | 面神经 神经再生 神经元型一氧化氮合酶 鼠 |
Expression of Neuronal Nitric Oxide Synthase in the Facial Nucleus after Facial Nerve Injury |
| |
| Affiliation: | WANG Peng, ZANG Xiao-yan, ZHANG Yin-cheng(1. Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University, Shanghai 200072; 2. Department of Oral and Maxillofacial Surgery, School of Stomatology, X i ＇ an Jiaotong University, Xi ＇an 710004, Shaanxi Province, China) |
| |
| Abstract: | Objective： To observe the variation of neuronal nitric oxide synthase （nNOS） expression in injured facial nerve tissue. Method： 20 adult male SD rats were used in this study, among them, 5 rats were normal controls. In experiment group, a compression injury was applied at one branch of bilateral facial nerve. Every 3 rats in experiment group were sacrified for nNOS immunostaining in a period of 1, 2, 3, 4 and 5 weeks respectively. Results： In normal facial nerve tissue, nNOS expression was not detected. In the injured facial nerve neurons, nNOS immunostaining expression was positive, and lasting up till the fifth weeks. Conclusion： After the nerve injury, the expressing of nNOS was observed in the facial nucleus, which may be related to the death of neuron. |
| |
| Keywords: | facial never nerve regeneration neuronal nitric oxide synthase rats |
| 本文献已被 维普 等数据库收录！ |
|
