大鼠MSCs的体外分离、培养及鉴定的实验研究

目的]体外分离、培养及鉴定大鼠骨髓间充质干细胞（MSCs）。方法]密度梯度离心法体外分离、培养7周龄sD大鼠MSCs，利用差速贴壁原理纯化MSCs，并通过形态学观察、细胞表面抗原标志物、多细胞系诱导分化对其进行鉴定。结果]第3代（P3代）不表达抗原CD34，表达抗原CD44，符合MSCs表面标志物特征。结论]密度梯度离心法能够建立稳定的MSCs体外分离培养体系。

Experimental study on isolation, culture and identification of MSCs in rats

Objective] To isolate, culture and purify bone marrow mesenchymal slem cells（MSCs）. Methods] MSCs from the rat bone marrow was obtained and purified in vitro by the method of gradient centritilgation combining with adherence cuhure. They were assess by immunoeytochemistry. Results] The samples of MSCs of third passage couht express CD44, but not CD34, according to the cbaracleristics of the MSCs. Conclusion] The method of density gradient centrifugation can establish a stable system to separate and culture MSCs in vitro.