3例SARS患者肺组织病理学观察及核转录因子-κB的表达

为观察严重急性呼吸综合征 (SARS)患者肺损伤的病理学特点及核转录因子 κB(NF κB)在肺组织中的表达 ,探讨急性肺损伤时NF κB的活化及其与肺损伤的关系 ,对 3例SARS死亡病例进行尸体解剖 ,HE染色观察肺组织的病理改变 ;采用免疫组织化学SP法检测肺组织NF κBp65活性。结果 :肺部病变主要表现为弥散性肺间质炎症及肺泡损伤 ,肺泡上皮细胞凋亡脱落及肺透明膜形成 ,肺间隔明显增宽 ,以淋巴细胞和单核细胞为主的炎细胞浸润。 3例肺组织NF κBp65检测均为阳性 ,阳性信号表达于肺泡上皮细胞、巨噬细胞及淋巴细胞的胞质及胞核内。结果提示 :SARS患者的肺组织表现为急性肺损伤 ,NF κB在SARS患者肺组织的损伤中起重要的作用

Expression of Nuclear Factor Kappa B(NF-κB) in Pneumonia of Severe Acute Respiratory Syndrome

In order to study the pathological characteristics of lungs in severe acute respiratory syndrome (SARS) and the expression of nuclear factor kappa B (NF-κB) in lungs, investigate the relationship between the activation of NF-κB and damage of lungs, HE staining and immuno-histochemical staining (SP methods) were used to study 3 lungs of autopsy cases of SARS.Antibody against NF-κB p65 was used for immunohistochemical studies.The pathological changes were diffuse pulmonary interstitial inflammation and alveolar damages with apoptosis and desquamation of pneumocytes in alveolar spaces, hyaline-membrane formation, wider alveolar walls and predominant lymphocyte and mononuclear inflammatory infiltrates.Immunoreactivity to NF-κBp65 were all positive and located in plasma and nuclei of pneumocytes, macrophages and lymphocytes.This study indicates that the lungs of patients with SARS show acute injury, NF-κB plays an important role in it.