HER2/neu胞外区片段基因免疫诱导特异细胞免疫及其抗瘤效应研究 *

目的：了解鱼精蛋白应用于血管内皮生长因子受体介导的靶向性非病毒载体的可行性。方法：ＣＶ１，ＣＶ２靶向性非病毒载体来比较多聚赖氨酸与鱼精蛋白对靶向性基因转移复合体携带ＤＮＡ的能力及体外基因转移效率的影响。结果：在Ａ３７５细胞中，鱼业 白与多聚赖氨酸参与形成的复合基因导入率都为５０％左右。在ＡＢＡＥ细胞中，鱼精蛋白参与形成的复合体基因导入率只有２０％左右，而多聚赖酸可达７０％左右。鱼精蛋白参与形成的复

Induction of Cellular Immune Response by Intramuscular DNA Immunization with HER-2/neu Extracellular Domain Gene and Its Anti-Tumor Effect

To study the cellular immune response and the anti-tumor effects induced by intramuscular DNA immunization with HER2/neu extracellular domain gene. Methods: HER2/neu oncogene extracellular domain gene was isolated and inserted to pCDNA3 expression vector. The cellular immune response and its anti-tumor effects were analyzed after intramuscular DNA immunization. Results: A specific cytotoxic activity by spleen cells from HER2/neu ECD gene immunized mice was found in vitro. Tumor growth was inhibited after tumor challenge in vivo in immunized mice. Conclusion: Specific cellular immune response and anti-tumor effect were elicited by HER2/neu extracellular domain gene immunization.