Pyk2在胃癌组织中的表达及其意义

目的:研究Pyk2(proline-rich tyrosine kinase-2)在正常胃黏膜组织与胃癌组织中表达的差异,探讨其意义.方法:应用免疫组织化学的方法检测59例正常胃黏膜组织标本和52例胃癌组织标本中Pyk2的表达情况.结果:免疫组织化学结果显示,Pyk2在正常胃黏膜组织中的表达阳性率为86.44%(51/59),在胃癌组织中表达阳性率为19.23%(10/52),两者差异具有统计学意义(P＜0.05);在高分化胃癌中表达阳性率为47.37%(9/19),而在中、低分化胃癌中表达阳性率为3.03%(1/33),两者差异具有统计学意义(P＜0.05).Pyk2表达阳性率在不同TNM分期胃癌中分别为:Ⅰ期66.67%(6/9),Ⅱ期30%(3/10),Ⅲ期3.45%(1/29),Ⅳ期0%(0/4),差异具有统计学意义[(Ⅱ Ⅲ Ⅳ)vI,x2=15.767,P＜0.05)].结论:本研究观察到Pyk2在正常胃黏膜组织中有表达,在胃癌组织中低表达或几乎无表达,并且其表达随着胃癌恶性度和TNM分期的增加而逐渐降低.提示Pyk2可能在胃癌的发生、发展过程中起重要作用.

Expression of proline-rich tyrosine kinase-2 (Pyk2) in gastric carcinoma and its significance

OBJECTIVE: To investigate whether proline-rich tyrosine kinase-2 (Pyk2) is expressed differently in normal gastric mucosas and gastric carcinoma tissues and further to evaluate its significance. METHODS: Expressions of Pyk2 in 59 cases of normal gastric mucosas and 52 cases of gastric carcinoma tissues were analysed by immunohistochemical methods. RESULTS: Immunohistochemical studies showed that the positive rates of Pyk2 protein expression in normal gastric mucosas and gastric carcinoma tissues were 86.44% (51/59) and 19.23% (10/52) respectively. The difference between normal gastric mucosas and gastric carcinoma tissues was statistically significant (P<0.05). The positive rates of Pyk2 expression in highly differentiated gastric carcinoma and moderately/lowly differentiated gastric carcinoma were 47.37% (9/19) and 3.03% (1/33) respectively. Statistically significant differences (P<0.05) were observed in the levels of Pyk2 expression between highly differentiated gastric carcinoma and moderately/lowly differentiated gastric carcinoma. The positive rates of Pyk2 expression at different TNM stages gastric carcinoma were respectively: stage I 66.67% (6/9), stage II 30% (3/10), stage III 3.45% (1/29), stage IV 0% (0/4). The differences were statistically significant [(II+III+IV) v I, chi2=15.767, P<0.05]. CONCLUSION: In this study, we demonstrate that Pyk2 is expressed in normal gastric mucosas, whereas its expression declines significantly or almost disappears in gastric carcinoma tissues. The expression of Pyk2 progressively decreases with increasing grade of malignancy and TNM stages of gastric carcinoma. This phenomenon indicates that Pyk2 expression may be involved in the generation and development of gastric carcinoma.