丙型肝炎病毒核心蛋白激活肝门部胆管癌细胞血管内皮生长因子的表达

目的 探讨丙型肝炎病毒(HCV)核心蛋白对肝门部胆管癌生长和转移的影响。方法 首先构建表达HCV核心蛋白的真核表达载体pcDNA3-HCVcore,再将含有 pcDNA3-HCVcore的载体和不含 pcDNA3-HCV core的空载体分别导入肝门部胆管癌细胞株 QBC939中,RT-PCR和免疫组织化学检测 VEGF mRNA和蛋白表达。结果 重组质粒 pcDNA3-HCVcore在 QBC939细胞中有稳定表达;表达 HCV核心蛋白的细胞QBC939的 VEGF在蛋白水平和 mRNA水平较转染空载体的细胞QBC939明显升高。结论 HCV核心蛋白能激活VEGF表达,可能对肝门部胆管癌的生长和转移具有促进作用。

HCV core protein activates expression of vascular endothelial growth factor in hilar cholangiocarcinoma cells

Objective To study the effect of HCV core protein on the growth and metastasis of hilar cholangiocarcinoma. Methods The eukaryon expression vector pcDNA3-HCV core, which could express HCV core protein, was firstly Constructed. The vector with pcDNA3 - HCV core and the vector without pcDNA3-HCV core were transducted into hilar cholangiocarcinoma celi strain (QBC 939) separately. VEGF mRNA and VEGF protein were detected by RT-PCR and immunohistochemistry respectively. Results The recombinant plasmids with pcDNA3-HCV core were stably expressed in QBC 939 celis. The VEGF mRNA and VEGF protein expressed in those QBC 939 celis transducted with pcDNA3-HCV core were higher than that of QBC 939 celis without pcD-NA3-HCV core. Conclusion HCV core protein can activate the expression of VEGF in QBC 939 celis and might contribute to the growth and metastasis of hilar cholangiocarcinoma.