|
|||||
|
|
| 青蒿琥酯对人子宫内膜癌细胞RL95-2的生长抑制作用 | |
| 引用本文： | 郑军生,王明华,黄敏,骆云鹏,米粲.青蒿琥酯对人子宫内膜癌细胞RL95-2的生长抑制作用[J].南方医科大学学报,2008,28(12):2221-2223. |
| 作者姓名： | 郑军生 王明华 黄敏 骆云鹏 米粲 |
| 作者单位： | 1. 中山大学附属第三医院妇产科,广东,广州,510630 2. 重庆医科大学病理教研室,重庆,400016 3. 重庆医科大学病理生理教研室,重庆,400016 |
| 摘 要： | 目的 研究青蒿琥酯在体外对人子宫内膜癌RL95-2细胞的生长抑制作用及其可能机制.方法 通过MTT法实验观察青蒿琥酯在体外对RL95-2细胞的抑制作用;透射电镜观察癌细胞的形态学改变;免疫组化检测细胞浆Caspase3活性;流式细胞术观察青蒿琥酯对细胞周期的影响及检测细胞线粒体膜电位△ψm和细胞内ROS水平.结果 青蒿琥酯对RL95-2细胞具有增殖抑制作用,其半数细胞抑制浓度(IC50)为26.29 μg/ml.透射电镜见:细胞呈早期凋亡改变:核染色质聚集于核膜周围,呈团块状;免疫组化检测细胞浆Caspase3表达阳性;流式细胞仪检测:细胞周期阻滞发生在G0/G1期;细胞内ROS升高(P<0.05);细胞线粒体膜电位△ψm降低(P<0.05).结论 青蒿琥酯在体外作用于人子宫颈癌RL95-2细胞,可能通过诱导细胞凋亡抑制癌细胞增殖. |
| 关 键 词： | 青蒿琥酯 人子宫内膜癌细胞株RL95-2 氧自由基 线粒体膜电位 细胞凋亡 |
Artesunate suppresses human endometrial carcinoma RL95-2 cell proliferation by inducing cell apoptosis |
|
| ZHENG Jun-sheng,WANG Ming-hua,HUANG Min,LUO Yun-peng,MI Can.Artesunate suppresses human endometrial carcinoma RL95-2 cell proliferation by inducing cell apoptosis[J].Journal of Southern Medical University,2008,28(12):2221-2223. | |
| Authors: | ZHENG Jun-sheng WANG Ming-hua HUANG Min LUO Yun-peng MI Can |
| Institution: | ZHENG Jun-sheng1,WANG Ming-hua2,HUANG Min1,LUO Yun-peng3,MI Can2 1Department of Gynecology , Obstetrics,Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China,2Department of Pathology,3Department of Pathophysiology,Chongqing Medical University,Chongqing 400016 |
| Abstract: | Objective To investigate the inhibitory effect of artesunate on human endometrial carcinoma RL95-2 cell line proliferation in vitro and the possible mechanisms. Methods The inhibitory effect of artesunate on the cell proliferation was assessed with MTT assay. Transmission electron miscrosopy was used to observe the morphological change of the cells after the treatment. Flow cytometry was performed to examine the changes in the cell cycle, reactive oxygen species (ROS) levels (with DCFH-DA labeling) and mito... |
| Keywords: | artesunate RL95-2 cell line reactive oxygen species mitochondrial member potential apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《南方医科大学学报》浏览原始摘要信息 | |
| 点击此处可从《南方医科大学学报》下载免费的PDF全文 | |