Application of bromodeoxyuridine immunohistochemistry to the rat temporomandibular joint. |
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| Authors: | J Sekine K Sano R Hironaka T Inokuchi |
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| Affiliation: | Second Department of Oral and Maxillofacial Surgery, Nagasaki University, School of Dentistry, Japan. |
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| Abstract: | To detect S-phase cells in the decalcified paraffin-embedded temporomandibular joint (TMJ) tissues of the rat, bromodeoxyuridine (BrdUrd) immunohistochemistry was performed. Percardiac perfusion of 10% EDTA-2Na Tris buffer accelerated the decalcification. The use of the monoclonal antibody to BrdUrd enabled the visualization of the distribution of S-phase cells. This method is practical in studying cell kinetics and the specific proliferation mode of the TMJ. |
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