扁桃体恶性肿瘤中microRNA-21和98的表达及临床意义

摘要：目的  初步探讨microRNA-21（miRNA-21）和miRNA-98在扁桃体恶性肿瘤的发生、发展及转移中的作用和临床意义。方法  应用实时荧光定量聚合酶链反应检测53例扁桃体恶性肿瘤组织,50例扁桃体炎症组织及20例正常组织中miRNA-21和miRNA-98的表达水平,并分析其在不同临床病理指标下的表达。结果  miRNA-21在扁桃体恶性肿瘤组织中的表达量高于扁桃体炎症组织及正常组织,差异有统计学意义（P <0.05）。恶性肿瘤组织中miRNA-21的表达量与临床分期和淋巴结转移有关,与病理分级无关。miRNA-98在扁桃体恶性肿瘤组织中的表达量低于扁桃体炎症组织及正常组织,差异有统计学意义（P <0.05）。结论  扁桃体恶性肿瘤的发生、发展及转移可能与miRNA-21和miRNA-98的表达密切相关。

Expressions of microRNA-21 and microRNA-98 in malignant tumors of tonsils and clinical significance

Abstract: Objective To investigate the role and clinical significance of the expressions of microRNA-21 (miRNA-21) and microRNA-98 (miRNA-98) in development and progression of malignant tonsilar tumors. Methods The expressions of miRNA-21 and miRNA-98 were detected by qRT-PCR in the 53 specimens of tonsilar neoplasms and 50 specimens of inflammatory tonsilar tissues and 20 specimens of normal muscosa. The expressions of miRNA-21 and miRNA-98 in the tonsilar neoplasms were analyzed with different clinicopathological parameters. Results The expression of miRNA-21 in the malignant tonsilar tumors was significantly higher than that in the inflammatory tonsilar tissues and normal muscosa (P < 0.05). There were positive correlations between miRNA-21 expression and both TNM staging and lymph node metastasis; but not pathological grade. The expression of miRNA-98 in the malignant tonsilar neoplasms was significantly lower than that in the inflammatory tonsilar tissues and normal muscosa (P < 0.05). Conclusions The expressions of miRNA-21 and miRNA-98 may be correlated with the development and progression of malignant tonsilar neoplasms.