AEG1与钙黏附蛋白E在食管癌中表达的相关性及其意义

目的  研究星形细胞上调基因1（AEG1）和钙黏附蛋白E（E-cad）在食管癌中的表达的相关性，并分析与临床病理特征的关系。方法  采用免疫组织化学方法和Western blot两种方法检测58例食管癌组织及其相应的癌旁组织中AEG1和钙黏附蛋白E的表达，并分析与临床病理特征的相关性。结果  在58例食管癌组织中AEG1表达的阳性率82.7%明显高于相应的癌旁组织的51.7%（P <0.05），且表达阳性率与性别、年龄、肿瘤的部位、肿瘤病理类型无相关性（P >0.05），而与肿瘤分化程度、浸润深度、临床分期、淋巴结转移相关（P < 0.05）；在58例食管癌组织中钙黏附蛋白E的阳性率为31%，明显低于相应癌旁组织的94.8%（P <0.05），且表达阳性率与性别、年龄、肿瘤的部位、肿瘤病理类型无相关性（P >0.05），而与肿瘤分化程度、浸润深度、临床分期、淋巴结转移相关（P <0.05）；食管癌中AEG1与钙黏附蛋白E的表达呈明显的负相关（r =-0.483，P <0.05）。结论  AEG1和钙黏附蛋白E在食管癌中的表达分别表现为高表达和表达缺失，且两者与食管癌的侵润、转移密切相关，AEG1可能介导食管癌中钙黏附蛋白E的表达下调。

Correlationship and significance of AEG1 and expression of E-cad in esophageal carcinoma

Objective To explore the correlationship of astrocyte elevated gene 1 (AEG1) and expression of E-cadherin (E-cad) protein in esophageal carcinoma, and analyze its relationship with clinical pathological features. Methods Immunohistochemical and Western Blot test methods were used to detect the expression of AEG1 and E-cadherin protein in 58 cases of esophageal carcinoma tissues and its corresponding adjacent tissues. Then we analyzed the relationship of AEG1, E-cadherin and clinicopathologic correlation. Results In 58 cases of esophageal carcinoma tissues, the positive expression rate of AEG1 was 82.7 %; The positive expression rate of AEG1 was 51.7 % in the corresponding adjacent tissues, the positive expression rate of AEG1 in esophageal carcinoma tissues was obviously higher than that in the corresponding adjacent tissues (P < 0.05), and the expression positive rate of AEG1 in esophageal carcinoma tissues was related to differentiated degree, depth of invasion, clinical stage, and lymph node metastasis (P < 0.05); but no relation with sex, age, tumor site, and pathologic types (P > 0.05); in 58 cases of esophageal carcinoma tissues, the positive expression rate of E-cadherin protein was 31%, the positive expression rate of E-cadherin was 94.8%; the positive expression rate of E-cadherin in esophageal carcinoma tissues was obviously less than that in the corresponding adjacent tissues (P < 0.05); It is related to differentiated degree, depth of invasion, clinical stage, and lymph node metastasis (P < 0.05); but no relation with sex, age, tumor site, and pathologic types (P > 0.05); the expression of AEG1 and E-cadherin protein was significantly negative correlation(r = 0.483, P < 0.05). Conclusions The expression of AEG1 and E-cadherin in esophageal carcinoma was characterized by over-expression and loss-expression, and they were closely associated with invasion and metastasis of esophageal carcinoma, AEG1 may be mediated E-cadherin less-expression in esophageal carcinoma.