果酸联合调q激光治疗中波紫外线诱导黄褐斑鼠模型的实验研究

目的 研究果酸联合调q激光治疗中波紫外线诱导的黄褐斑鼠模型的疗效及可能机制。方法 选取健康雌性豚鼠30只，分为正常组（6只）和实验组（24只）。实验组予中波紫外线联合黄体酮注射液复制黄褐斑鼠模型，从中随机选取6只与正常组豚鼠一起处死，观察血清超氧化物歧化酶（SOD）和丙二醛(MDA)的表达水平，局部皮损行HE及免疫组织化学染色。实验组余18只为模型组，随机分为A、B、C组，每组6只。A组予以果酸联合调q激光治疗，B组予以氢醌治疗，C组不做任何处理。4个月后分别检测各组豚鼠血清、肝脏、皮肤SOD活力、MDA表达水平及皮肤组织病理改变。结果 模型组黄褐斑鼠模型复制区可见深褐色斑形成；模型组与正常组比较，血清SOD水平下降，MDA水平上升（P?<0.05）；模型组皮损HE及免疫组织化学染色显示病理表现符合黄褐斑病理改变；A、B组与C组比较，A组、B组皮损肉眼观及组织病理表现明显改善；治疗后血清、肝脏、皮肤中SOD水平上升，MDA水平下降（P?<0.05），A组与B组比较，差异无统计学意义（P?>0.05）。结论 果酸联合调q激光治疗黄褐斑鼠疗效显著，可能与果酸的剥脱作用、抗氧化作用，以及调q激光选择性破坏黑素细胞、减少黑色素生成有关。

Pathological experimental study of alpha hydroxy acid combined with Q-switched laser in the treatment of chloasma mouse model induced by UVB

Objective To explore the effect and the possible mechanism of alpha hydroxy acid combined with Q-switched laser in the treatment of melasma mouse model induced by UVB. Methods Totally 30 healthy female guinea pigs were selected and were randomly divided into normal group (6 guinea pigs) and model group (24 guinea pigs). The model group was given UVB combined with progesterone to induced chloasma mode. 6 model mice were randomly selected to observe the levels of both serum superoxide dismutase (SOD) and malondialdehyde (MDA) and the pathology of skin tissues by HE staining and immunohistochemistry. The normal group was handled as the same. The rest of the 18 mice in the model group were randomly divided into group A, group B and group C. Group A was treated with a combination of alpha hydroxy acid combined with Q-switched laser, group B was treated with hydroquinone, group C was done with nothing. After 4 months, the levels of SOD and MDA in serum, liver and skin lesions and the pathology of skin tissue were detected. Results Dark brown spot formed in the molding bay of model group; the expression levels of serum SOD in the model group was clearly lower, and the expression levels of serum MDA was higher than those of the normal group (P??0.05). Conclusion The curative effect of alpha hydroxy acid combined with Q-switched laser in melasma mouse model was significantly, which may be related to the exfoliation and antioxidation of alpha hydroxy acid and the selective destruction of Q-switched Laser for melanocyte.