高迁移率族蛋白A2 与原发性肝细胞癌患者 临床病理特征及其预后的关系

目的 探讨高迁移率族蛋白A2（HMGA2）表达与原发性肝细胞癌患者临床病理特征及其预后 的关系。方法 选取2010 年1 月—2013 年1 月在西南医科大学附属医院肝胆外科行手术切除的肝细胞癌患 者120 例。以免疫组织化学法检测患者手术切除标本的癌组织、癌旁组织及正常肝组织中HMGA2 的表达， 记录患者临床病理特征并随访。统计分析HMGA2 阳性表达与患者临床病理特征的关系，以及对患者预后的 影响。结果 免疫组织化学结果发现，HMGA2 阳性表达呈棕黄色或棕褐色颗粒，主要定位于肿瘤细胞的细 胞核。肝癌组织HMGA2 阳性率比正常肝组织和癌旁组织高（P <0.05）。HMGA2 阳性表达率在临床分期、 有肝门淋巴结转移及肝内转移肝细胞癌患者中较高（P <0.05）。多因素Logistic 回归模型结果显示，临床分期、 肝门淋巴结转移及肝内转移均是影响HMGA2 阳性表达的独立危险因素（P <0.05）。两组患者的3 年生存率 比较，差异有统计学意义（P <0.05）。结论 HMGA2 阳性患者预后明显较差，HMGA2 可作为判断肝细胞癌 患者预后的一个潜在指标。

Association of HMGA2 expression with clinicopathological parameters and prognosis in primary hepatocellular carcinoma

Objective To investigate the relationship of HMGA2 expression with clinicopathological parameters and prognosis in primary hepatocellular carcinoma. Methods The clinical data of 120 patients with hepatocellular carcinoma who underwent surgical resection from January 2010 to January 2013 in our hospital were collected. Immunohistochemical method was used to detect the expression of HMGA2 protein in cancer tissues, adjacent tissues and normal liver tissues of the resected specimens, and the clinicopathological parameters were recorded and followed up. The relationship between the positive expression of HMGA2 protein and clinical pathological parameters and prognosis of the patie nts was analyzed. Results Immunohistochemical staining showed that the positive expression of HMGA2 protein was brown or tan, mainly located in the nuclei of tumor cells. The positive rate of HMGA2 protein in HCC tissues was higher than in adjacent tissues and normal liver tissues (P < 0.05). The expression of HMGA2 protein was higher in patients with hepatocellular carcinoma in high clinical stage, hilar lymph node metastasis and intrahepatic metastasis (P < 0.05). Multivariate analysis showed that clinical stage, hepatic hilar lymph node metastasis and intrahepatic metastasis were independent risk factors of HMGA2 protein expression (P < 0.05). The difference of 3-year survival rate between the two groups was statistically significant (P < 0.05). Conclusions The prognosis of patients with positive HMGA2 protein is poor, and HMGA2 protein can be used as a potential index to judge the prognosis of patients with HCC.