15个产地玄参中哈巴苷与哈巴俄苷含量测定

目的 建立HPLC法同时测定玄参中哈巴苷、哈巴俄苷的含量.方法 采用HPLC-PDA法测定,固定相采用Eclipse Plus C18色谱柱,以乙腈–0.03％磷酸水溶液为流动相,梯度洗脱;体积流量1.0 ml/min;检测波长210、280 nm.结果 哈巴苷、哈巴俄苷分别在0.1020～0.5100 mg/ml(r...

Determination of harpagide and harpagoside contents in Scrophularia ningpoensis from 15 origins

Objective To establish an HPLC method for the simultaneous determination of harpagide and harpagoside content in Scrophularia ningpoensis (SN).Methods An Eclipse C₁₈ column was used for determination of methanol extract of S. ningpoensis with a HPLC-PDA method and mobile phase of acetonitrile-0.03% phosphate solution in a gradient elution manner. The flow rate of mobile phase was 1.0 ml/min, and the detection wavelengths were 210 nm and 280 nm.Results Harpagide and harpagoside contents in SN showed good linear relationships within 0.1020-0.5100 mg/ml (r=0.9999) and 0.0340-0.1700 mg/ml (r=0.9999). Their average recovery rates were 97.44% and 97.08%.The RSDs were 0.93% and 1.24%.. There were significant differences in the contents of harpagide and harpagoside in SN from 15 origins (P<0.01). The content of harpagoside in Sichuan Long-dong, Zhejiang Lin-an, Zhejiang Pan-an and Henan Nan-feng is higher.Conclusion This method is stable, accurate and reproducible and can be used for the quality control of SN.