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Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus
Authors:Izumiya Hidemasa  Matsumoto Kazutoshi  Yahiro Shunsuke  Lee Jiyoung  Morita Masatomo  Yamamoto Shouji  Arakawa Eiji  Ohnishi Makoto
Affiliation:aDepartment of Bacteriology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan;bKumamoto Prefectural Institute of Public-Health and Environmental Science, 1240-1 Kurizakimachi, Uto City, Kumamoto 869-0425, Japan
Abstract:A multiplex PCR assay was developed based on atpA-sequence diversification for molecular identification of 3 major pathogenic Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. It specifically identified them from among 133 strains of various Vibrio species and other genera, and was applicable for testing seawater, suggesting its usefulness.
Keywords:atpA   Multiplex PCR   V.   cholerae   V.   parahaemolyticus   V.   vulnificus
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