Distribution of PDE8A in the nervous system of the Sprague-Dawley rat |
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Authors: | Kruse Lars Schack Møller Morten Kruuse Christina |
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Affiliation: | aDepartment of Neurology, Glostrup Hospital, University of Copenhagen, Nordre Ringvej 57, DK-2600 Glostrup, Denmark;bDepartment of Neuroscience and Pharmacology, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark |
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Abstract: | Phosphodiesterases (PDEs) are essential regulators of cyclic nucleotide signaling. Little is known of the distribution and function of the cyclic adenosine monophosphate (cAMP) hydrolyzing PDE8A family.Employing immunohistochemistry and Western blots this study maps the distribution of PDE8A in the brain of adult male Sprague-Dawley rats and in the trigeminal ganglion.PDE8A was confined to neuronal perikaryal cytoplasm and to processes extending from those perikarya. The neurons exhibiting PDE8A-immunoreactivity were widely distributed in the forebrain, brain stem, and cerebellum. Strongly immunoreactive neurons were located in the olfactory bulb, the septal area, zona incerta, and reticular nucleus of the thalamus. Less immunoreactivity was seen in the hippocampus and cerebral cortex. Intense staining was detected in both the substantia nigra and the sensory trigeminal nucleus. In cerebellum PDE8A immunoreactivity was located not only in the Purkinje cells, but also in the granular cells as well as the parallel fibres in the molecular layer. PDE8A immunoreactivity was represented in the epithelial lining of the choroids plexus, the dura mater, and the neurons of the trigeminal ganglion.The localization of the cAMP degrading PDE8A may indicate a role for PDE8A in cAMP signaling related to pain transmission, motor function, cognition and olfaction. |
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Keywords: | Abbreviations: AM, amygdala AOP, anterior olfactory nucleus pars, posterior Ap, area postrema CA1, CA1 region of hippocampal gyrus Gr, nucleus gracilis Gi, gigantocellular reticular nucleus Cu, nucleus cuneatus DC, dorsal cochlear nucleus DLG, dorsal nucleus of the lateral geniculate nucleus EPl, external plexiforme layer of olfactory bulb Fr, frontal neocortex fr, fasciculus retroflexus GP, globus pallidus HDB, diagonal band of Broca, horizontal part ic, internal capsule icp, inferior cerebellar peduncle Ion, inferior olivary nucleus Igsc, intermediate gray layer of superior colliculus IMM, intermediomedial cell column of lamina VII Ipn, interpeduncular nucleus Lar, lateral reticular nucleus lo, lateral olfactory tract Mo, motor cortex Mrn, medullary reticular nucleus MS, medial septal nucleus Mt, mamillothalamic tract NX, nervus vagus Och, optic chiasm Opt, optic tract PAG, periaqueductal gray PBG, parabigeminal nucleus Pc, posterior commissure Pir, piriform cortex Pn, pontine reticular nucleus Rn, red nucleus Sc, superior colliculus Sn, substantia nigra Soln, solitary nucleus sp5, spinal trigeminal tract Sp5n, spinal trigeminal nucleus Rt, reticular nucleus STR, striatum VC, ventral cochlear nucleus VDB, diagonal band of Broca, vertical part |
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