| 柯萨奇病毒B_3致病模型的建立 |
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| 引用本文： | 申元英,杨占秋,刘建军,肖红,徐芳玲,文利,毛琳.柯萨奇病毒B_3致病模型的建立[J].武汉大学学报(医学版),2001,22(4):297-299. |
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| 作者姓名： | 申元英 杨占秋 刘建军 肖红 徐芳玲 文利 毛琳 |
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| 作者单位： | 武汉大学医学院病毒学研究所， |
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| 基金项目： | 湖北省教育厅资助课题,批准号:98A051 |
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| 摘 要： | 目的 :建立柯萨奇病毒 (CV)B3 的实验动物模型 ,以期为抗病毒药物筛选提供工具。方法 :雄性BALB/C鼠腹腔注射CVB3 后 ,观察小鼠发病症状 ,并在不同时间处死小鼠 ,取小鼠心、脾、肝、肾等组织进行病毒分离、病毒核酸检测、心肌组织病理检查。结果 :感染后第 3d小鼠即出现症状 ,第 5d在小鼠的心脏、脾脏、肾脏均可分离出感染性病毒 ,其滴度分别为 :10 4 .7,10 3.3 ,10 3.2 TCID50 / 0 .1ml,同时小鼠心、脾、肾、等组织及血清中CVB RNA检测为阳性 ,心肌组织出现典型炎性改变。结论 :雄性BALB/C鼠感染CVB3 后可引起病毒性心肌炎发生
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| 关 键 词： | 柯萨奇病毒B组 心肌炎 模型 生物学 小鼠 BALB/C |
| 修稿时间： | 2000年11月17 |
Pathological Observation of Infection of Coxsakievirus B3 |
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| Shen Yuan ying,Yang Zhan qiu,Liu Jian Jun,et al Institute of Virology,Medical College,Wuhan University,Wuhan ,China.Pathological Observation of Infection of Coxsakievirus B3[J].Medical Journal of Wuhan University,2001,22(4):297-299. |
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| Authors: | Shen Yuan ying Yang Zhan qiu Liu Jian Jun Institute of Virology Medical College Wuhan University Wuhan China |
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| Institution: | Shen Yuan ying,Yang Zhan qiu,Liu Jian Jun,et al Institute of Virology,Medical College,Wuhan University,Wuhan 430071,China |
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| Abstract: | Abstracts: To set up a pathogenic model in mice that infected with coxsackievirus B 3. Methods: CVB 3 was inoculated introperitonally in male BALB/C mouse.And then symptoms were observed, CVB 3 was isolated and viral RNA was detected in murine hearts, spleens, livers and the pathological lesion was detected. Results: most of mouse appeared symptoms in the 3rd day postinfection,and CVB 3 was isolated at 10 4.7 ,10 3.3 ,10 3.2 TCID 50 /0.1 ml in hearts; spleens; kidneys respectively; CVB RNA were positive in hearts, spleens, kidneys and the sera of the mice at 5th day and 10th day postinfection.Conclusion: The viral myocarditis was developed in mice which could be a model for screening anti CVB agents. |
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| Keywords: | coxackie B viruses myocarditis models biological mice inbred balb/c |
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