金丝桃苷体外抗胃癌作用及其机制研究

目的：研究金丝桃苷（Hyperoside）对胃癌BGC-823细胞的生长、周期及凋亡的作用,并通过检测半胱氨酸天冬氨酸蛋白酶（caspase 3、caspase 8、caspase 9）的活性探讨金丝桃苷诱导胃癌凋亡的可能机制。方法：采用MTT法检测金丝桃苷对胃癌BGC-823细胞的增殖影响;流式细胞技术检测金丝桃苷对胃癌BGC-823细胞周期和凋亡的影响;比色法测定金丝桃苷对胃癌BGC-823细胞凋亡过程中caspase 3、caspase 8和caspase 9活性影响。结果：金丝桃苷处理BGC-823细胞24h和48h后,细胞生长明显抑制,作用24h和48h时其IC50分别为32.14和22.67μg/mL;金丝桃苷处理组与对照组相比,胃癌BGC-823细胞G0/G1期比例明显增加,凋亡细胞比例明显增加;caspase 3、caspase 8和caspase9活性随药物浓度增加而逐渐升高。结论：金丝桃苷能够通过阻断细胞周期、诱导细胞凋亡有效抗胃癌,其诱导肿瘤细胞凋亡机制可能与激活caspase 3、caspase 8和caspase 9活性密切相关。

Anticancer effects of hyperoside on human gastric cancer cells and its mechanisms

AIM. To observe the effects of hyperoside on the cell proliferation, cycle and apoptosis of human gastric cancer cell line BGC- 823 and to explore its possible mechanisms. METHODS： The effects of hyperoside on the proliferation of BGC-823 cells were evaluated by MTT assay. Flow cytometry was used to analyze the cell cycle and apoptosis of the cells exposed to hyperoside. The activities of caspase 3, caspase 8 and caspase 9 were examined by spectrophotometry. RESULTS： With the hyperoside incubation for 24 h or 48 h resulted in a significant inhibition of proliferation in BGC-823 cells as a ICs0 of 32.14/lg/mL and 22.67 /lg/mL. After treatment with hyperoside,the cell cycle was suppressed obviously at Go/G1 phase. The apoptotic rates were significantly higher in the cells treated with hyperoside than in the control. Comparing with the control, the activities of caspase 3, caspase 8 and caspase 9 were up-regu- lated in hyperoside group. CONCLUSION： Hyperoside have significant anti-gastric tumor effects by induce cell cycle arrest and apoptosis of BGC-823 cells, and intracelular caspase 3, caspase 8 and caspase 9 are c with the apoptosis. osely associated